Developmental Biology

Anti-ING2 Antibody (CAB12266)

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SKU:
CAB12266
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-ING2 Antibody
Antibody SKU:CAB12266
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-180 of human ING2 (NP_001555.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:A-549, MCF7, HT-29, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-180 of human ING2 (NP_001555.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RSRL LTCY VQDY LECV ESLP HDMQ RNVS VLRE LDNK YQET LKEI DDVY EKYK KEDD LNQK KRLQ QLLQ RALI NSQE LGDE KIQI VTQM LELV ENRA RQME LHSQ CFQD PAES ERAS DKAK MDSS QPER SSRR PRRQ RTSE SRDL CHMA NGIE DCDD QPPK E
Gene ID:3622
Uniprot:Q9H160
Cellular Location:Nucleus
Calculated MW:27kDa/32kDa
Observed MW:33kDa
Synonyms:ING2, ING1L, p33ING2
Background:This gene is a member of the inhibitor of growth (ING) family. Members of the ING family associate with and modulate the activity of histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes and function in DNA repair and apoptosis. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:ING2: Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs). Belongs to the ING family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: CCAAT-binding factor complex; cytoplasm; Golgi apparatus; nucleoplasm; nucleus; plasma membrane; Sin3 complex

Molecular Function:DNA binding; methylated histone residue binding; phosphoinositide binding; protein binding; protein complex binding

Biological Process: chromatin modification; male germ-line stem cell division; male meiosis I; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of transcription, DNA-dependent; signal transduction; sperm motility; spermatid development; spermatogenesis

NCBI Summary:This gene is a member of the inhibitor of growth (ING) family. Members of the ING family associate with and modulate the activity of histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes and function in DNA repair and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
UniProt Code:Q9H160
NCBI GenInfo Identifier:59798471
NCBI Gene ID:3622
NCBI Accession:Q9H160.2
UniProt Secondary Accession:Q9H160,O95698, B6ZDS1,
UniProt Related Accession:Q9H160
Molecular Weight:27,984 Da
NCBI Full Name:Inhibitor of growth protein 2
NCBI Synonym Full Names:inhibitor of growth family member 2
NCBI Official Symbol:ING2  
NCBI Official Synonym Symbols:ING1L; p33ING2  
NCBI Protein Information:inhibitor of growth protein 2
UniProt Protein Name:Inhibitor of growth protein 2
UniProt Synonym Protein Names:Inhibitor of growth 1-like protein; ING1Lp; p32; p33ING2
Protein Family:Inhibitor of growth protein
UniProt Gene Name:ING2  
UniProt Entry Name:ING2_HUMAN
Anti-ING2 Antibody (CAB12266)Western blot analysis of extracts of various cell lines, using ING2 antibody (CAB12266) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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