Antibody Name:	Anti-IMPA1 Antibody
Antibody SKU:	CAB6381
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human IMPA1 (NP_005527.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SKOV3, SW620, Jurkat, SH-SY5Y, Mouse liver, Mouse kidney, Mouse brain, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human IMPA1 (NP_005527.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MADP WQEC MDYA VTLA RQAG EVVC EAIK NEMN VMLK SSPV DLVT ATDQ KVEK MLIS SIKE KYPS HSFI GEES VAAG EKSI LTDN PTWI IDPI DGTT NFVH RFPF VAVS IGFA VNKK IEFG VVYS CVEG KMYT ARKG KGAF CNGQ KLQV SQQE DITK SLLV TELG SSRT PETV RMVL SNME KLFC IPVH GIRS VGTA AVNM CLVA TGGA DAYY EMGI HCWD VAGA GIIV TEAG GVLM DVTG GPFD LMSR RVIA ANNR ILAE RIAK EIQV IPLQ RDDE D
Gene ID:	3612
Uniprot:	P29218
Cellular Location:	Cytoplasm
Calculated MW:	21kDa/30kDa/36kDa
Observed MW:	30kDa

Synonyms:

IMPA1, IMP, IMPA, MRT59

Background:

This gene encodes an enzyme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1, 4, 5-trisphosphate and diacylglycerol. This enzyme can also use myo-inositol-1, 3-diphosphate, myo-inositol-1, 4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. This enzyme shows magnesium-dependent phosphatase activity and is inhibited by therapeutic concentrations of lithium. Inhibition of inositol monophosphate hydroylosis and subsequent depletion of inositol for phosphatidylinositol synthesis may explain the anti-manic and anti-depressive effects of lithium administered to treat bipolar disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms. A pseudogene of this gene is also present on chromosome 8q21.13.

UniProt Protein Function:	IMPA1: Responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides and has been implicated as the pharmacological target for lithium action in brain. Can use myo-inositol monophosphates, myo-inositol 1,3-diphosphate, myo-inositol 1,4-diphosphate, scyllo-inositol- phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1- phosphate, beta-glycerophosphate, and 2'-AMP as substrates. Belongs to the inositol monophosphatase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Phosphatase (non-protein); Carbohydrate Metabolism - inositol phosphate; EC 3.1.3.94; EC 3.1.3.25 Chromosomal Location of Human Ortholog: 8q21.13-q21.3 Cellular Component: cytoplasm; cytosol Molecular Function:identical protein binding; protein homodimerization activity; manganese ion binding; inositol-1(or 4)-monophosphatase activity; magnesium ion binding; lithium ion binding Biological Process: inositol phosphate metabolic process; phosphoinositide phosphorylation; inositol phosphate dephosphorylation; inositol biosynthetic process; phosphatidylinositol biosynthetic process; signal transduction; phosphate metabolic process
NCBI Summary:	This gene encodes an enzyme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1,4,5-trisphosphate and diacylglycerol. This enzyme can also use myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. This enzyme shows magnesium-dependent phosphatase activity and is inhibited by therapeutic concentrations of lithium. Inhibition of inositol monophosphate hydroylosis and subsequent depletion of inositol for phosphatidylinositol synthesis may explain the anti-manic and anti-depressive effects of lithium administered to treat bipolar disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms. A pseudogene of this gene is also present on chromosome 8q21.13. [provided by RefSeq, Dec 2014]
UniProt Code:	P29218
NCBI GenInfo Identifier:	127717
NCBI Gene ID:	3612
NCBI Accession:	P29218.1
UniProt Secondary Accession:	P29218,Q9UK71, B2R733, B4DLN3, B7Z6Q4, J3KQT7,
UniProt Related Accession:	P29218
Molecular Weight:	277
NCBI Full Name:	Inositol monophosphatase 1
NCBI Synonym Full Names:	inositol(myo)-1(or 4)-monophosphatase 1
NCBI Official Symbol:	IMPA1
NCBI Official Synonym Symbols:	IMP; IMPA
NCBI Protein Information:	inositol monophosphatase 1; IMP 1; IMPase 1; inositol-1(or 4)-monophosphatase 1; D-galactose 1-phosphate phosphatase; lithium-sensitive myo-inositol monophosphatase A1
UniProt Protein Name:	Inositol monophosphatase 1
UniProt Synonym Protein Names:	D-galactose 1-phosphate phosphatase
Protein Family:	Inositol monophosphatase
UniProt Gene Name:	IMPA1
UniProt Entry Name:	IMPA1_HUMAN

	Western blot analysis of extracts of various cell lines, using IMPA1 antibody (CAB6381) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using IMPA1 antibody (CAB6381) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using IMPA1 antibody (CAB6381) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using IMPA1 antibody (CAB6381) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of A549 cells using IMPA1 antibody (CAB6381). Blue: DAPI for nuclear staining.