Cell Biology Antibodies 8
Anti-IMMT Antibody (CAB2751)
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- SKU:
- CAB2751
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-IMMT Antibody |
| Antibody SKU: | CAB2751 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-270 of human IMMT (NP_006830.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | MCF7, B cells, HeLa, HepG2, SH-SY5Y, HL-60, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-270 of human IMMT (NP_006830.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ESKQ PASQ LQKQ KGDT PASA TAPT EAAQ IISA AGDT LSVP APAV QPEE SLKT DHPE IGEG KPTP ALSE EASS SSIR ERPP EEVA ARLA QQEK QEQV KIES LAKS LEDA LRQT ASVT LQAI AAQN AAVQ AVNA HSNI LKAA MDNS EIAG EKK |
| Gene ID: | 10989 |
| Uniprot: | Q16891 |
| Cellular Location: | Mitochondrion, Mitochondrion inner membrane, Single-pass membrane protein |
| Calculated MW: | 80kDa/82kDa/83kDa |
| Observed MW: | 83kDa |
| Synonyms: | IMMT, HMP, MINOS2, Mic60, P87, P87/89, P89, PIG4, PIG52 |
| Background: |
| UniProt Protein Function: | IMMT: 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial; Membrane protein, integral Chromosomal Location of Human Ortholog: 2p11.2|2 Cellular Component: mitochondrion; membrane; mitochondrial inner membrane; integral to membrane Molecular Function:protein binding Biological Process: mitochondrial calcium ion homeostasis; response to cold |
| UniProt Code: | Q16891 |
| NCBI GenInfo Identifier: | 29427676 |
| NCBI Gene ID: | |
| NCBI Accession: | Q16891.1 |
| Molecular Weight: | ~ 84kDa |
| NCBI Full Name: | MICOS complex subunit MIC60 |
| UniProt Protein Name: | MICOS complex subunit MIC60 |
| UniProt Synonym Protein Names: | Cell proliferation-inducing gene 4/52 protein; Mitochondrial inner membrane protein; Mitofilin; p87/89 |
| UniProt Gene Name: | IMMT |
| UniProt Entry Name: | MIC60_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IMMT antibody (CAB2751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Confocal immunofluorescence analysis of U2OS cells using IMMT Polyclonal Antibody (CAB2751) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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