Immunology Antibodies 2
Anti-ILF3 Antibody (CAB2496)
- SKU:
- CAB2496
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-ILF3 Antibody |
| Antibody SKU: | CAB2496 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ILF3 (NP_036350.2). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, HepG2, Jurkat, U-87MG, mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ILF3 (NP_036350.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MRPM RIFV NDDR HVMA KHSS VYPT QEEL EAVQ NMVS HTER ALKA VSDW IDEQ EKGS SEQA ESDN MDVP PEDD SKEG AGEQ KTEH MTRT LRGV MRVG LVAK GLLL KGDL DLEL VLLC KEKP TTAL LDKV ADNL AIQL AAVT EDKY EILQ SVDD AAIV IKNT KEPP LSLT IHLT SPVV REEM EKVL AGET LSVN DPPD VLDR QKCL AALA SLRH AKWF QARA NGLK SCVI VIRV LRDL CTRV PTWG PLRG WPLE LLCE KSIG TANR PMGA GE |
| Gene ID: | 3609 |
| Uniprot: | Q12906 |
| Cellular Location: | Cytoplasm, Nucleus, nucleolus |
| Calculated MW: | 74kDa/75kDa/76kDa/82kDa/95kDa |
| Observed MW: | 95, 120kDa |
| Synonyms: | ILF3, CBTF, DRBF, DRBP76, MMP4, MPHOSPH4, MPP4, NF-AT-90, NF110, NF110b, NF90, NF90a, NF90b, NFAR, NFAR-1, NFAR2, TCP110, TCP80 |
| Background: | This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | NFAT90: a double-stranded RNA (dsRNA)-binding protein implicated in the regulation of gene expression. Phosphorylated in a double-stranded RNA-dependent manner possibly by PKR. Five different splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:RNA-binding; Transcription factor; DNA-binding; Nucleolus Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: nucleoplasm; membrane; mitochondrion; cytoplasm; nucleolus; ribonucleoprotein complex; nucleus Molecular Function:protein binding; DNA binding; RNA binding; double-stranded RNA binding Biological Process: transcription, DNA-dependent; negative regulation of viral genome replication; positive regulation of transcription, DNA-dependent; negative regulation of translation; negative regulation of transcription, DNA-dependent; defense response to virus; protein amino acid phosphorylation |
| UniProt Code: | Q12906 |
| NCBI GenInfo Identifier: | 62512150 |
| NCBI Gene ID: | |
| NCBI Accession: | Q12906.3 |
| Molecular Weight: | |
| NCBI Full Name: | Interleukin enhancer-binding factor 3 |
| UniProt Protein Name: | Interleukin enhancer-binding factor 3 |
| UniProt Synonym Protein Names: | Double-stranded RNA-binding protein 76; DRBP76; M-phase phosphoprotein 4; MPP4; Nuclear factor associated with dsRNA; NFAR; Nuclear factor of activated T-cells 90 kDa; NF-AT-90; Translational control protein 80; TCP80 |
| Protein Family: | Interleukin enhancer-binding factor |
| UniProt Gene Name: | ILF3 |
| UniProt Entry Name: | ILF3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ILF3 antibody (CAB2496) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Western blot analysis of extracts of mouse thymus, using ILF3 antibody (CAB2496) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of C6 cells using ILF3 antibody (CAB2496) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH-3T3 cells using ILF3 antibody (CAB2496) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using ILF3 antibody (CAB2496) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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