Cell Biology Antibodies 12
Anti-IL5RA Antibody (CAB8552)
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- SKU:
- CAB8552
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-IL5RA Antibody |
| Antibody SKU: | CAB8552 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-335 of human IL5RA (NP_783855.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60, B cells, U-87MG, Mouse liver, Mouse lung, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-335 of human IL5RA (NP_783855.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ADLL PDEK ISLL PPVN FTIK VTGL AQVL LQWK PNPD QEQR NVNL EYQV KINA PKED DYET RITE SKCV TILH KGFS ASVR TILQ NDHS LLAS SWAS AELH APPG SPGT SIVN LTCT TNTT EDNY SRLR SYQV SLHC TWLV GTDA PEDT QYFL YYRY GSWT EECQ EYSK DTLG RNIA CWFP RTFI LSKG RDWL AVLV NGSS KHSA IRPF DQLF ALHA IDQI NPPL NVTA EIEG TRLS IQWE KPVS AFPI HCFD YEVK IHNT RNGY LQIE KLMT NAFI SIID DLSK YDVQ VRAA VSSM CREA GLWS EWSQ PIYV GFSR |
| Gene ID: | 3568 |
| Uniprot: | Q01344 |
| Cellular Location: | Membrane, Single-pass type I membrane protein |
| Calculated MW: | 23kDa/37kDa/42kDa/47kDa |
| Observed MW: | 76kDa |
| Synonyms: | IL5RA, CD125, CDw125, HSIL5R3, IL5R |
| Background: | The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported. |
| UniProt Protein Function: | IL5RA: This is the receptor for interleukin-5. The alpha chain binds to IL5. Belongs to the type I cytokine receptor family. Type 5 subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Receptor, cytokine Chromosomal Location of Human Ortholog: 3p26-p24 Cellular Component: extracellular space; plasma membrane Molecular Function:interleukin-5 receptor activity; protein binding; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity Biological Process: cell proliferation; MAPKKK cascade; signal transduction |
| NCBI Summary: | The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported. [provided by RefSeq, Jul 2011] |
| UniProt Code: | Q01344 |
| NCBI GenInfo Identifier: | 116242525 |
| NCBI Gene ID: | 3568 |
| NCBI Accession: | Q01344.2 |
| UniProt Secondary Accession: | Q01344,Q14633, Q15469, Q6ISX9, B3IU77, B4E2G0, |
| UniProt Related Accession: | Q01344 |
| Molecular Weight: | 23,737 Da |
| NCBI Full Name: | Interleukin-5 receptor subunit alpha |
| NCBI Synonym Full Names: | interleukin 5 receptor subunit alpha |
| NCBI Official Symbol: | IL5RA |
| NCBI Official Synonym Symbols: | IL5R; CD125; CDw125; HSIL5R3 |
| NCBI Protein Information: | interleukin-5 receptor subunit alpha |
| UniProt Protein Name: | Interleukin-5 receptor subunit alpha |
| UniProt Synonym Protein Names: | CDw125; CD_antigen: CD125 |
| Protein Family: | Interleukin-5 receptor |
| UniProt Gene Name: | IL5RA |
| UniProt Entry Name: | IL5RA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IL5RA antibody (CAB8552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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