Antibody Name:	IL7 Rabbit Polyclonal Antibody
Antibody SKU:	CAB1650
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 26-177 of human IL7 (NP_000871.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 26-177 of human IL7 (NP_000871.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DCDI EGKD GKQY ESVL MVSI DQLL DSMK EIGS NCLN NEFN FFKR HICD ANKE GMFL FRAA RKLR QFLK MNST GDFD LHLL KVSE GTTI LLNC TGQV KGRK PAAL GEAQ PTKS LEEN KSLK EQKK LNDL CFLK RLLQ EIKT CWNK ILMG TKEH
Gene ID:	3574
Uniprot:	P13232
Cellular Location:	Secreted
Calculated MW:	7kDa/15kDa/20kDa
Observed MW:	17kDa

Synonyms:

IL7, IL-7

Background:

The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.

UniProt Protein Function:	IL7: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. Belongs to the IL-7/IL-9 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Apoptosis; Secreted, signal peptide; Cell development/differentiation; Cytokine; Secreted; Cell cycle regulation Chromosomal Location of Human Ortholog: 8q12-q13 Cellular Component: extracellular space; extracellular region Molecular Function:interleukin-7 receptor binding; protein binding; growth factor activity; cytokine activity Biological Process: positive regulation of organ growth; humoral immune response; T cell lineage commitment; organ morphogenesis; positive regulation of T cell differentiation; cell-cell signaling; regulation of gene expression; homeostasis of number of cells within a tissue; negative regulation of catalytic activity; positive regulation of cell proliferation; positive regulation of B cell proliferation; negative regulation of apoptosis; bone resorption
NCBI Summary:	The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.[provided by RefSeq, Dec 2010]
UniProt Code:	P13232
NCBI GenInfo Identifier:	124354
NCBI Gene ID:	3574
NCBI Accession:	P13232.1
UniProt Secondary Accession:	P13232,Q5FBY5, Q5FBY9, A0N0L3,
UniProt Related Accession:	P13232
Molecular Weight:	177
NCBI Full Name:	Interleukin-7
NCBI Synonym Full Names:	interleukin 7
NCBI Official Symbol:	IL7
NCBI Official Synonym Symbols:	IL-7
NCBI Protein Information:	interleukin-7
UniProt Protein Name:	Interleukin-7
Protein Family:	Interleukin
UniProt Gene Name:	IL7
UniProt Entry Name:	IL7_HUMAN

	Western blot analysis of extracts of THP-1 cells, using IL7 antibody (CAB1650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded rat liver using IL7 antibody (CAB1650) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using IL7 antibody (CAB1650) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using IL7 antibody (CAB1650). Blue: DAPI for nuclear staining.