Immunology Antibodies 1
Anti-IL-6 Antibody (CAB0286)
- SKU:
- CAB0286
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | IL6 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB0286 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-212 of human IL6 (NP_000591.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Mouse heart, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 30-212 of human IL6 (NP_000591.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VPPG EDSK DVAA PHRQ PLTS SERI DKQI RYIL DGIS ALRK ETCN KSNM CESS KEAL AENN LNLP KMAE KDGC FQSG FNEE TCLV KIIT GLLE FEVY LEYL QNRF ESSE EQAR AVQM STKV LIQF LQKK AKNL DAIT TPDP TTNA SLLT KLQA QNQW LQDM TTHL ILRS FKEF LQSS LRAL RQM |
| Gene ID: | 3569 |
| Uniprot: | P05231 |
| Cellular Location: | Secreted |
| Calculated MW: | 23kDa |
| Observed MW: | 27kDa |
| Synonyms: | IL6, BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6 |
| Background: | This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. |
| UniProt Code: | P05231 |
| NCBI GenInfo Identifier: | 124347 |
| NCBI Gene ID: | 3569 |
| NCBI Accession: | P05231.1 |
| UniProt Secondary Accession: | P05231,Q9UCU2, Q9UCU3, Q9UCU4, |
| UniProt Related Accession: | P05231 |
| Molecular Weight: | 212 |
| NCBI Full Name: | Interleukin-6 |
| NCBI Synonym Full Names: | interleukin 6 |
| NCBI Official Symbol: | IL6 |
| NCBI Official Synonym Symbols: | HGF; HSF; BSF2; IL-6; IFNB2 |
| NCBI Protein Information: | interleukin-6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; interferon, beta 2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor |
| UniProt Protein Name: | Interleukin-6 |
| UniProt Synonym Protein Names: | B-cell stimulatory factor 2; BSF-2; CTL differentiation factor; CDF; Hybridoma growth factor; Interferon beta-2; IFN-beta-2 |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL6 |
| UniProt Entry Name: | IL6_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IL6 antibody (CAB0286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat spleen using IL6 Rabbit pAb (CAB0286) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using IL6 Rabbit pAb (CAB0286) at dilution of 1:100 (40x lens). |
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