Antibody Name:	IL5 Rabbit Polyclonal Antibody
Antibody SKU:	CAB7690
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-134 of human IL5 (NP_000870.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse thymus, Mouse liver, Rat thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-134 of human IL5 (NP_000870.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IPTE IPTS ALVK ETLA LLST HRTL LIAN ETLR IPVP VHKN HQLC TEEI FQGI GTLE SQTV QGGT VERL FKNL SLIK KYID GQKK KCGE ERRR VNQF LDYL QEFL GVMN TEWI IES
Gene ID:	3567
Uniprot:	P05113
Cellular Location:	Secreted
Calculated MW:	15kDa
Observed MW:	13kDa

Synonyms:

IL5, EDF, IL-5, TRF

Background:

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

UniProt Protein Function:	IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide; Cytokine Chromosomal Location of Human Ortholog: 5q31.1 Cellular Component: extracellular region; extracellular space; intracellular Molecular Function:cytokine activity; growth factor activity; interleukin-5 receptor binding; protein binding Biological Process: activation of MAPKK activity; axon guidance; cytokine and chemokine mediated signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; inflammatory response; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; nerve growth factor receptor signaling pathway; positive regulation of B cell proliferation; positive regulation of eosinophil differentiation; positive regulation of immunoglobulin secretion; positive regulation of JAK-STAT cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription factor activity; Ras protein signal transduction; small GTPase mediated signal transduction; vascular endothelial growth factor receptor signaling pathway
NCBI Summary:	This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]
UniProt Code:	P05113
NCBI GenInfo Identifier:	124341
NCBI Gene ID:	3567
NCBI Accession:	P05113.1
UniProt Secondary Accession:	P05113,Q13840,
UniProt Related Accession:	P05113
Molecular Weight:	15,238 Da
NCBI Full Name:	Interleukin-5
NCBI Synonym Full Names:	interleukin 5
NCBI Official Symbol:	IL5
NCBI Official Synonym Symbols:	EDF; TRF; IL-5
NCBI Protein Information:	interleukin-5
UniProt Protein Name:	Interleukin-5
UniProt Synonym Protein Names:	B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF
Protein Family:	Interleukin
UniProt Gene Name:	IL5
UniProt Entry Name:	IL5_HUMAN

	Western blot analysis of extracts of various cell lines, using IL5 antibody (CAB7690) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded human liver using IL5 antibody (CAB7690) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using IL5 antibody (CAB7690) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
AF/LE Purified Human/Mouse IL-5 Monoclonal Antibody [TRFK5](AGEL2062)	Human IL-5 CLIA Kit (HUES00165)
Human/Mouse IL-5 Monoclonal Antibody (PE Conjugated) [TRFK5](AGEL2063)	Human IL-5 ELISA Kit (HUES01458)
Human/Mouse IL-5 Monoclonal Antibody (APC Conjugated) [TRFK5](AGEL2064)	Human IL-5 ELISA Kit (HUEB0028)
Human/Mouse IL-5 Monoclonal Antibody (PE Conjugated) [TRFK5](AGEL2065)
Human/Mouse IL-5 Monoclonal Antibody (APC Conjugated) [TRFK5](AGEL2066)