Immunology Antibodies 1
Anti-IL-4R Antibody (CAB14648)
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- SKU:
- CAB14648
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | IL4R Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB14648 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-232 of human IL4R (NP_000409.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562, A-431, 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-232 of human IL4R (NP_000409.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKVL QEPT CVSD YMSI STCE WKMN GPTN CSTE LRLL YQLV FLLS EAHT CIPE NNGG AGCV CHLL MDDV VSAD NYTL DLWA GQQL LWKG SFKP SEHV KPRA PGNL TVHT NVSD TLLL TWSN PYPP DNYL YNHL TYAV NIWS ENDP ADFR IYNV TYLE PSLR IAAS TLKS GISY RARV RAWA QCYN TTWS EWSP STKW HNSY REPF EQH |
| Gene ID: | 3566 |
| Uniprot: | P24394 |
| Cellular Location: | Cell membrane, Secreted, Single-pass type I membrane protein |
| Calculated MW: | 25kDa/88kDa/89kDa |
| Observed MW: | 140kDa |
| Synonyms: | IL4R, CD124, IL-4RA, IL4RA |
| Background: | This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants. |
| UniProt Protein Function: | Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. |
| NCBI Summary: | This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012] |
| UniProt Code: | P24394 |
| NCBI GenInfo Identifier: | 124335 |
| NCBI Gene ID: | 3566 |
| NCBI Accession: | P24394.1 |
| UniProt Secondary Accession: | P24394,Q96P01, Q9H181, Q9H182, Q9H183, Q9H184, Q9H185 Q9H186, Q9H187, B4E076, B9EKU8, H3BSY5, |
| UniProt Related Accession: | P24394 |
| Molecular Weight: | 88,267 Da |
| NCBI Full Name: | Interleukin-4 receptor subunit alpha |
| NCBI Synonym Full Names: | interleukin 4 receptor |
| NCBI Official Symbol: | IL4R |
| NCBI Official Synonym Symbols: | CD124; IL4RA; IL-4RA |
| NCBI Protein Information: | interleukin-4 receptor subunit alpha |
| UniProt Protein Name: | Interleukin-4 receptor subunit alpha |
| UniProt Synonym Protein Names: | CD_antigen: CD124 |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL4R |
 | Western blot analysis of extracts of various cell lines, using IL4R antibody (CAB14648) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat spleen using IL4R antibody (CAB14648) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using IL4R antibody (CAB14648) at dilution of 1:100 (40x lens). |
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