Immunology Antibodies 2
Anti-IL-27 Antibody (CAB5498)
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- SKU:
- CAB5498
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | IL27 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB5498 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 29-243 of human IL27 (NP_663634.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | A-549, HeLa, 293T, HepG2, 22Rv1, Mouse spleen, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 29-243 of human IL27 (NP_663634.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FPRP PGRP QLSL QELR REFT VSLH LARK LLSE VRGQ AHRF AESH LPGV NLYL LPLG EQLP DVSL TFQA WRRL SDPE RLCF ISTT LQPF HALL GGLG TQGR WTNM ERMQ LWAM RLDL RDLQ RHLR FQVL AAGF NLPE EEEE EEEE EEEE RKGL LPGA LGSA LQGP AQVS WPQL LSTY RLLH SLEL VLSR AVRE LLLL SKAG HSVW PLGF PTLS PQP |
| Gene ID: | 246778 |
| Uniprot: | Q8NEV9 |
| Cellular Location: | Secreted |
| Calculated MW: | 27kDa |
| Observed MW: | 55kDa |
| Synonyms: | IL27, IL-27, IL-27A, IL27A, IL27p28, IL30, p28 |
| Background: | The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR). |
| UniProt Protein Function: | IL27-alpha: Cytokine with pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon- gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK- dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL-27 subunit p28 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP- 10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replivation. Belongs to the IL-6 superfamily. |
| UniProt Protein Details: | Protein type:Cytokine; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 16p11 Cellular Component: extracellular space Molecular Function:interleukin-27 receptor binding; cytokine activity; receptor binding Biological Process: regulation of T-helper 1 cell differentiation; response to bacterium; regulation of T cell proliferation; regulation of defense response to virus; innate immune response; inflammatory response; positive regulation of interferon-gamma biosynthetic process |
| NCBI Summary: | The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR). [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8NEV9 |
| NCBI GenInfo Identifier: | 182701368 |
| NCBI Gene ID: | 246778 |
| NCBI Accession: | Q8NEV9.2 |
| UniProt Secondary Accession: | Q8NEV9,Q6P676, A0N0L2, |
| UniProt Related Accession: | Q8NEV9 |
| Molecular Weight: | 27,493 Da |
| NCBI Full Name: | Interleukin-27 subunit alpha |
| NCBI Synonym Full Names: | interleukin 27 |
| NCBI Official Symbol: | IL27 |
| NCBI Official Synonym Symbols: | p28; IL30; IL-27; IL27A; IL-27A; IL27p28 |
| NCBI Protein Information: | interleukin-27 subunit alpha; IL27-A; IL-27-A; interleukin 30; interleukin-30; IL-27 p28 subunit; IL-27 subunit alpha |
| UniProt Protein Name: | Interleukin-27 subunit alpha |
| UniProt Synonym Protein Names: | Interleukin-30; p28 |
| Protein Family: | Interleukin |
| UniProt Gene Name: | IL27 |
| UniProt Entry Name: | IL27A_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IL27 antibody (CAB5498) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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