Antibody Name:	IL12RB1 Rabbit Polyclonal Antibody
Antibody SKU:	CAB13319
Antibody Size:	20uL, 50uL, 100uL
Application:	IHC IF
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-305 of human IL12RB1 (NP_714912.1).

Application:	IHC IF
Recommended Dilution:	IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-305 of human IL12RB1 (NP_714912.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RTSE CCFQ DPPY PDAD SGSA SGPR DLRC YRIS SDRY ECSW QYEG PTAG VSHF LRCC LSSG RCCY FAAG SATR LQFS DQAG VSVL YTVT LWVE SWAR NQTE KSPE VTLQ LYNS VKYE PPLG DIKV SKLA GQLR MEWE TPDN QVGA EVQF RHRT PSSP WKLG DCGP QDDD TESC LCPL EMNV AQEF QLRR RQLG SQGS SWSK WSSP VCVP PENP PQPQ VRFS VEQL GQDG RRRL TLKE QPTQ LELP EGCQ GLAP GTEV TYRL QLHM LSCP CKAK ATRT LHLG K
Gene ID:	3594
Uniprot:	P42701
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	42kDa/72kDa/73kDa
Observed MW:

Synonyms:

IL12RB1, CD212, IL-12R-BETA1, IL12RB, IMD30

Background:

The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	IL12RB1: Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade. Defects in IL12RB1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD); also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity, whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. Belongs to the type I cytokine receptor family. Type 2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Receptor, cytokine Chromosomal Location of Human Ortholog: 19p13.1 Cellular Component: external side of plasma membrane Molecular Function:hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-12 receptor activity; interleukin-23 receptor activity; interleukin-23 binding; interleukin-12 receptor binding Biological Process: positive regulation of memory T cell differentiation; positive regulation of interferon-gamma production; peptidyl-tyrosine phosphorylation; positive regulation of T-helper 1 type immune response; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; positive regulation of activated T cell proliferation; signal transduction; positive regulation of defense response to virus by host Disease: Immunodeficiency 30
NCBI Summary:	The protein encoded by this gene is a type I transmembrane protein that belongs to the hemopoietin receptor superfamily. This protein binds to interleukine 12 (IL12) with a low affinity, and is thought to be a part of IL12 receptor complex. This protein forms a disulfide-linked oligomer, which is required for its IL12 binding activity. The coexpression of this and IL12RB2 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. Mutations in this gene impair the development of interleukin-17-producing T lymphocytes and result in increased susceptibility to mycobacterial and Salmonella infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
UniProt Code:	P42701
NCBI GenInfo Identifier:	1170462
NCBI Gene ID:	3594
NCBI Accession:	P42701.1
UniProt Secondary Accession:	P42701,Q8N6Q7, A8K308, B2RPF1, B7ZKK3,
UniProt Related Accession:	P42701
Molecular Weight:	662
NCBI Full Name:	Interleukin-12 receptor subunit beta-1
NCBI Synonym Full Names:	interleukin 12 receptor, beta 1
NCBI Official Symbol:	IL12RB1
NCBI Official Synonym Symbols:	CD212; IMD30; IL12RB; IL-12R-BETA1
NCBI Protein Information:	interleukin-12 receptor subunit beta-1; IL-12R subunit beta-1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; cluster of differentiation 212; interleukin-12 receptor beta-1 chain
UniProt Protein Name:	Interleukin-12 receptor subunit beta-1
UniProt Synonym Protein Names:	IL-12 receptor beta component; CD_antigen: CD212
Protein Family:	Interleukin-12 receptor
UniProt Gene Name:	IL12RB1
UniProt Entry Name:	I12R1_HUMAN

	Immunohistochemistry of paraffin-embedded rat kidney using IL12RB1 antibody (CAB13319) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of MCF7 cells using IL12RB1 antibody (CAB13319). Blue: DAPI for nuclear staining.