Anti-IL-10RB Antibody (CAB15680)

Name: Anti-IL-10RB Antibody (CAB15680)
Brand: Immunology Antibodies 2
SKU: CAB15680
Availability: OutOfStock

Antibody Name:	IL10RB Rabbit Polyclonal Antibody
Antibody SKU:	CAB15680
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-220 of human IL10RB (NP_000619.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, 293T, Jurkat, mouse skeletal muscle, rat heart, rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-220 of human IL10RB (NP_000619.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVPP PENV RMNS VNFK NILQ WESP AFAK GNLT FTAQ YLSY RIFQ DKCM NTTL TECD FSSL SKYG DHTL RVRA EFAD EHSD WVNI TFCP VDDT IIGP PGMQ VEVL ADSL HMRF LAPK IENE YETW TMKN VYNS WTYN VQYW KNGT DEKF QITP QYDF EVLR NLEP WTTY CVQV RGFL PDRN KAGE WSEP VCEQ TTHD ETVP S
Gene ID:	3588
Uniprot:	Q08334
Cellular Location:	Membrane, Single-pass type I membrane protein
Calculated MW:	36kDa
Observed MW:	36kDa

Synonyms:

IL10RB, CDW210B, CRF2-4, CRFB4, D21S58, D21S66, IL-10R2

Background:

The protein encoded by this gene belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. This gene and three other interferon receptor genes, IFAR2, IFNAR1, and IFNGR2, form a class II cytokine receptor gene cluster located in a small region on chromosome 21.

UniProt Protein Function:	Shared cell surface receptor required for the activation of five class 2 cytokines: IL10, IL22, IL26, IL28, and IFNL1. The IFNLR1/IL10RB dimer is a receptor for the cytokine ligands IFNL2 and IFNL3 and mediates their antiviral activity. The ligand/receptor complex stimulate the activation of the JAK/STAT signaling pathway leading to the expression of IFN-stimulated genes (ISG), which contribute to the antiviral state.
NCBI Summary:	The protein encoded by this gene belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. This gene and three other interferon receptor genes, IFAR2, IFNAR1, and IFNGR2, form a class II cytokine receptor gene cluster located in a small region on chromosome 21. [provided by RefSeq, Jul 2008]
UniProt Code:	Q08334
NCBI GenInfo Identifier:	56757647
NCBI Gene ID:	3588
NCBI Accession:	Q08334.2
UniProt Secondary Accession:	Q08334,Q9BUU4,
UniProt Related Accession:	Q08334
Molecular Weight:	36,995 Da
NCBI Full Name:	Interleukin-10 receptor subunit beta
NCBI Synonym Full Names:	interleukin 10 receptor subunit beta
NCBI Official Symbol:	IL10RB
NCBI Official Synonym Symbols:	CRFB4; CRF2-4; D21S58; D21S66; CDW210B; IL-10R2
NCBI Protein Information:	interleukin-10 receptor subunit beta
UniProt Protein Name:	Interleukin-10 receptor subunit beta
UniProt Synonym Protein Names:	Cytokine receptor class-II member 4; Cytokine receptor family 2 member 4; CRF2-4; Interleukin-10 receptor subunit 2; IL-10R subunit 2; IL-10R2; CD_antigen: CDw210b
Protein Family:	Interleukin-10 receptor
UniProt Gene Name:	IL10RB

	Western blot analysis of extracts of various cell lines, using IL10RB antibody (CAB15680) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of L929 cells using IL10RB antibody (CAB15680) at dilution of 1:100. Blue: DAPI for nuclear staining.