Antibody Name:	Anti-IKBKAP Antibody
Antibody SKU:	CAB10127
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1093-1332 of human IKBKAP (NP_003631.2).

Application:	WB IHC
Recommended Dilution:	WB 1:1000 - 1:4000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HepG2, U-251MG, HL-60, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1093-1332 of human IKBKAP (NP_003631.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LRLV YKYN RLDI IETN VKPS ILEA QKNY MAFL DSQT ATFS RHKK RLLV VREL KEQA QQAG LDDE VPHG QESD LFSE TSSV VSGS EMSG KYSH SNSR ISAR SSKN RRKA ERKK HSLK EGSP LEDL ALLE ALSE VVQN TENL KDEV YHIL KVLF LFEF DEQG RELQ KAFE DTLQ LMER SLPE IWTL TYQQ NSAT PVLG PNST ANSI MASY QQQK TSVP VLDA ELFI PPKI NRRT QWKL SLLD
Gene ID:	8518
Uniprot:	O95163
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	150kDa
Observed MW:	150kDa

Synonyms:

IKBKAP, DYS, ELP1, FD, IKAP, IKI3, TOT1

Background:

The protein encoded by this gene is a scaffold protein and a regulator for three different kinases involved in proinflammatory signaling. The encoded protein can bind NF-kappa-B-inducing kinase and I-kappa-B kinases through separate domains and assemble them into an active kinase complex. Mutations in this gene have been associated with familial dysautonomia. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	IKBKAP: May act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK). Interacts preferentially with MAP3K14/NIK followed by IKK-alpha and IKK-beta. Component of the RNA polymerase II elongator complex (Elongator), which consists of IKBKAP/ELP1, STIP1/ELP2, ELP3, ELP4, and two yet unidentified proteins, p30 and p38. Elongator associates with the C-terminal domain (CTD) of Pol II largest subunit. Interacts with ELP3. Belongs to the ELP1/IKA1 family.
UniProt Protein Details:	Protein type:Transcription regulation; Protein kinase, regulatory subunit Chromosomal Location of Human Ortholog: 9q31 Cellular Component: transcription elongation factor complex; Elongator holoenzyme complex; cytoplasm; nucleolus; histone acetyltransferase complex Molecular Function:protein binding; signal transducer activity; phosphorylase kinase regulator activity Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; RNA elongation from RNA polymerase II promoter; immune response; protein complex assembly; signal transduction; regulation of protein kinase activity; protein amino acid phosphorylation; positive regulation of cell migration Disease: Neuropathy, Hereditary Sensory And Autonomic, Type Iii
NCBI Summary:	The protein encoded by this gene is a scaffold protein and a regulator for 3 different kinases involved in proinflammatory signaling. This encoded protein can bind NF-kappa-B-inducing kinase (NIK) and IKKs through separate domains and assemble them into an active kinase complex. Mutations in this gene have been associated with familial dysautonomia. [provided by RefSeq, Jul 2008]
UniProt Code:	O95163
NCBI GenInfo Identifier:	3757822
NCBI Gene ID:	8518
NCBI Accession:	AAC64258.1
UniProt Secondary Accession:	O95163,Q5JSV2, Q9H327, Q9UG87,
UniProt Related Accession:	O95163
Molecular Weight:	150,254 Da
NCBI Full Name:	IkappaB kinase complex associated protein
NCBI Synonym Full Names:	inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein
NCBI Official Symbol:	IKBKAP
NCBI Official Synonym Symbols:	FD; DYS; ELP1; IKAP; IKI3; TOT1
NCBI Protein Information:	elongator complex protein 1
UniProt Protein Name:	Elongator complex protein 1
UniProt Synonym Protein Names:	IkappaB kinase complex-associated protein; IKK complex-associated protein; p150
UniProt Gene Name:	IKBKAP
UniProt Entry Name:	ELP1_HUMAN

	Western blot analysis of extracts of various cell lines, using IKBKAP antibody (CAB10127) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human liver cancer using IKBKAP Rabbit pAb (CAB10127) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using IKBKAP Rabbit pAb (CAB10127) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat stomach using IKBKAP Rabbit pAb (CAB10127) at dilution of 1:100 (40x lens).