Immunology Antibodies 1

Anti-IkBAlpha Antibody (CAB11474)

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SKU:
CAB11474
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-IkBAlpha Antibody
Antibody SKU:CAB11474
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human IkBAlpha (NP_065390.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:DU145, HeLa, Mouse thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human IkBAlpha (NP_065390.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MFQA AERP QEWA MEGP RDGL KKER LLDD RHDS GLDS MKDE EYEQ MVKE LQEI RLEP QEVP RGSE PWKQ QLTE DGDS FLHL AIIH EEKA LTME VIRQ VKGD LAFL NFQN NLQQ TPLH LAVI TNQP EIAE ALLG AGCD PELR DFRG NTPL HLAC EQGC LASV GVLT QSCT TPHL HSIL KATN YNGH TCLH LASI HGYL GIVE LLVS LGAD VNAQ EPCN GRTA LHLA VDLQ NPDL VSLL LKCG ADVN RVTY QGYS PYQL TWGR PSTR IQQQ LGQL TLEN LQML PESE DEES YDTE SEFT EFTE DELP YDDC VFGG QRLT L
Gene ID:4792
Uniprot:P25963
Cellular Location:Cytoplasm, Nucleus
Calculated MW:35kDa
Observed MW:36kDa
Synonyms:IKBA, MAD-3, NFKBI, IKB alpha, NFKBIA, EDAID2
Background:This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
UniProt Protein Function:Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
NCBI Summary:This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
UniProt Code:P25963
NCBI GenInfo Identifier:126682
NCBI Gene ID:4792
NCBI Accession:P25963.1
UniProt Secondary Accession:P25963,B2R8L6,
UniProt Related Accession:P25963
Molecular Weight:35,609 Da
NCBI Full Name:NF-kappa-B inhibitor alpha
NCBI Synonym Full Names:NFKB inhibitor alpha
NCBI Official Symbol:NFKBIA  
NCBI Official Synonym Symbols:IKBA; MAD-3; NFKBI  
NCBI Protein Information:NF-kappa-B inhibitor alpha
UniProt Protein Name:NF-kappa-B inhibitor alpha
UniProt Synonym Protein Names:I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3
Protein Family:NF-kappa-B inhibitor
UniProt Gene Name:NFKBIA  
UniProt Entry Name:IKBA_HUMAN
Anti-IkBAlpha Antibody (CAB11474)Western blot analysis of extracts of various cell lines, using IkBalpha antibody (CAB11474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 25s.
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