Antibody Name:	Anti-IGF2BP1 Antibody
Antibody SKU:	CAB13581
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 480 to the C-terminus of human IGF2BP1 (NP_006537.3).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, K-562, HepG2, HeLa, NIH/3T3, A-549, HL-60, Mouse liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 480 to the C-terminus of human IGF2BP1 (NP_006537.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FGPK EEVK LETH IRVP ASAA GRVI GKGG KTVN ELQN LTAA EVVV PRDQ TPDE NDQV IVKI IGHF YASQ MAQR KIRD ILAQ VKQQ HQKG QSNQ AQAR RK
Gene ID:	10642
Uniprot:	Q9NZI8
Cellular Location:	Cell projection, Cytoplasm, Nucleus, axon, dendrite, dendritic spine, filopodium, growth cone, lamellipodium, perinuclear region
Calculated MW:	48kDa/63kDa
Observed MW:	70kDa

Synonyms:

IGF2BP1, CRD-BP, CRDBP, IMP-1, IMP1, VICKZ1, ZBP1

Background:

This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	IGF2BP1: a conserved trans-acting factor that can control translation of specific mRNAs in vivo. Contains four K homology (KH) domains and two RNA recognition motifs. It functions by binding to the 5' UTR of the target mRNAs and repressing translation. Target mRNAs include those encoding beta-actin and insulin-like growth factor 2 (IGF2). Tyrosine phosphorylation apparently abolishes translational repression of actin expression by decreasing its binding beta-actin mRNA. May have a role in progression of ovarian cancer. Contains nuclear export signals within the RNA-binding KH2 and KH4 domains. Aberrant expression may interfere with c-myc regulation.
UniProt Protein Details:	Protein type:RNA-binding; Translation Chromosomal Location of Human Ortholog: 17q21.32 Cellular Component: growth cone; perinuclear region of cytoplasm; stress granule; axon; lamellipodium; cytoplasm; dendritic spine; ribonucleoprotein complex; cytosol; nucleus; filopodium Molecular Function:mRNA binding; protein binding; mRNA 3'-UTR binding; nucleotide binding; translation regulator activity; mRNA 5'-UTR binding Biological Process: mRNA transport; negative regulation of translation; gene expression; regulation of cytokine biosynthetic process
NCBI Summary:	This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
UniProt Code:	Q9NZI8
NCBI GenInfo Identifier:	296434536
NCBI Gene ID:	10642
NCBI Accession:	Q9NZI8.2
UniProt Related Accession:	Q9NZI8
Molecular Weight:
NCBI Full Name:	Insulin-like growth factor 2 mRNA-binding protein 1
NCBI Synonym Full Names:	insulin like growth factor 2 mRNA binding protein 1
NCBI Official Symbol:	IGF2BP1
NCBI Official Synonym Symbols:	IMP1; ZBP1; CRDBP; IMP-1; CRD-BP; VICKZ1
NCBI Protein Information:	insulin-like growth factor 2 mRNA-binding protein 1
UniProt Protein Name:	Insulin-like growth factor 2 mRNA-binding protein 1
UniProt Synonym Protein Names:	Coding region determinant-binding protein; CRD-BP; IGF-II mRNA-binding protein 1; VICKZ family member 1; Zipcode-binding protein 1; ZBP-1
UniProt Gene Name:	IGF2BP1
UniProt Entry Name:	IF2B1_HUMAN

	Western blot analysis of extracts of various cell lines, using IGF2BP1 antibody (CAB13581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug IGF2BP1 antibody (CAB13581). Western blot was performed from the immunoprecipitate using IGF2BP1 antibody (CAB13581) at a dilition of 1:1000.