Immunology Antibodies 1

Anti-IFIT3 Antibody (CAB13317)

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SKU:
CAB13317
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-IFIT3 Antibody
Antibody SKU:CAB13317
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 291-490 of human IFIT3 (NP_001540.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:DU145, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 291-490 of human IFIT3 (NP_001540.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QMQN TGES EASG NKEM IEAL KQYA MDYS NKAL EKGL NPLN AYSD LAEF LETE CYQT PFNK EVPD AEKQ QSHQ RYCN LQKY NGKS EDTA VQHG LEGL SISK KSTD KEEI KDQP QNVS ENLL PQNA PNYW YLQG LIHK QNGD LLQA AKCY EKEL GRLL RDAP SGIG SIFL SASE LEDG SEEM GQGA VSSS PREL LSNS EQLN
Gene ID:3437
Uniprot:O14879
Cellular Location:Cytoplasm, Mitochondrion
Calculated MW:55kDa
Observed MW:60kDa
Synonyms:IFIT3, CIG-49, GARG-49, IFI60, IFIT4, IRG2, ISG60, P60, RIG-G, cig41
Background:
UniProt Protein Function:IFIT3: IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS- mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exihibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin- dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2. Belongs to the IFIT family.
UniProt Protein Details:

Protein type:Unknown function

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: mitochondrion; cytoplasm; cytosol

Molecular Function:identical protein binding; protein binding

Biological Process: negative regulation of cell proliferation; cytokine and chemokine mediated signaling pathway; response to virus; defense response to virus; negative regulation of apoptosis

UniProt Code:O14879
NCBI GenInfo Identifier:6831570
NCBI Gene ID:3437
NCBI Accession:O14879.1
UniProt Secondary Accession:O14879,Q99634, Q9BSK7,
UniProt Related Accession:O14879
Molecular Weight:490
NCBI Full Name:Interferon-induced protein with tetratricopeptide repeats 3
NCBI Synonym Full Names:interferon-induced protein with tetratricopeptide repeats 3
NCBI Official Symbol:IFIT3  
NCBI Official Synonym Symbols:P60; IRG2; IFI60; IFIT4; ISG60; RIG-G; CIG-49; GARG-49  
NCBI Protein Information:interferon-induced protein with tetratricopeptide repeats 3; CIG49; IFIT-3; IFIT-4; ISG-60; IFI-60K; interferon-induced 60 kDa protein; retinoic acid-induced gene G protein; interferon-induced protein with tetratricopeptide repeats 4
UniProt Protein Name:Interferon-induced protein with tetratricopeptide repeats 3
UniProt Synonym Protein Names:CIG49; ISG-60; Interferon-induced 60 kDa protein; IFI-60K; Interferon-induced protein with tetratricopeptide repeats 4; IFIT-4; Retinoic acid-induced gene G protein; P60; RIG-G
Protein Family:Interferon-induced protein with tetratricopeptide repeats
UniProt Gene Name:IFIT3  
UniProt Entry Name:IFIT3_HUMAN
Anti-IFIT3 Antibody (CAB13317)Western blot analysis of extracts of various cell lines, using IFIT3 antibody (CAB13317) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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