Immunology Antibodies 3

Anti-IFIT1 Antibody (CAB8551)

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SKU:
CAB8551
Product Type:
Antibody
Applications:
WB
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

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Antibody Name:Anti-IFIT1 Antibody
Antibody SKU:CAB8551
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 301-388 of human IFIT1 (NP_001539.3).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse heart, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 301-388 of human IFIT1 (NP_001539.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:QIKE ATKG QPRG QNRE KLDK MIRS AIFH FESA VEKK PTFE VAHL DLAR MYIE AGNH RKAE ENFQ KLLC MKPV VEET MQDI HFHY GRFQ
Gene ID:3434
Uniprot:P09914
Cellular Location:Cytoplasm
Calculated MW:51kDa/55kDa
Observed MW:48kDa
Synonyms:IFIT1, C56, G10P1, IFI-56, IFI-56K, IFI56, IFIT-1, IFNAI1, ISG56, P56, RNM561
Background:This gene encodes a protein containing tetratricopeptide repeats that was originally identified as induced upon treatment with interferon. The encoded protein may inhibit viral replication and translational initiation. This gene is located in a cluster on chromosome 10 with five other closely related genes. There is a pseudogene for this gene on chromosome 13. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
UniProt Protein Function:IFIT1: IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O- methylation (cap snatching mechanism). Exhibits antiviral activity against several viruses including human papilloma and hepatitis C viruses. Belongs to the IFIT family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 10q23.31

Cellular Component: cytoplasm; cytosol

Molecular Function:protein binding; RNA binding

Biological Process: cytokine and chemokine mediated signaling pathway; defense response to virus; intracellular transport of viral proteins in host cell; negative regulation of defense response to virus by host; negative regulation of helicase activity; negative regulation of protein binding; negative regulation of viral genome replication; positive regulation of viral genome replication; response to virus

NCBI Summary:This gene encodes a protein containing tetratricopeptide repeats that was originally identified as induced upon treatment with interferon. The encoded protein may inhibit viral replication and translational initiation. This gene is located in a cluster on chromosome 10 with five other closely related genes. There is a pseudogene for this gene on chromosome 13. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Aug 2012]
UniProt Code:P09914
NCBI GenInfo Identifier:400286381
NCBI Gene ID:3434
NCBI Accession:NP_001257857.1
UniProt Secondary Accession:P09914,Q5T7J1, Q96QM5, B3KS50, D3DR31,
UniProt Related Accession:P09914
Molecular Weight:51,713 Da
NCBI Full Name:interferon-induced protein with tetratricopeptide repeats 1 isoform 3
NCBI Synonym Full Names:interferon induced protein with tetratricopeptide repeats 1
NCBI Official Symbol:IFIT1  
NCBI Official Synonym Symbols:C56; P56; G10P1; IFI56; ISG56; IFI-56; IFIT-1; IFNAI1; RNM561; IFI-56K  
NCBI Protein Information:interferon-induced protein with tetratricopeptide repeats 1
UniProt Protein Name:Interferon-induced protein with tetratricopeptide repeats 1
UniProt Synonym Protein Names:Interferon-induced 56 kDa protein; IFI-56K; P56
Protein Family:Interferon-induced protein with tetratricopeptide repeats
UniProt Gene Name:IFIT1  
UniProt Entry Name:IFIT1_HUMAN
Anti-IFIT1 Polyclonal Antibody (CAB8551)Western blot analysis of extracts of various cell lines, using IFIT1 antibody (CAB8551) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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