Antibody Name:	Anti-IDS Antibody
Antibody SKU:	CAB1857
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 34-290 of human IDS (NP_006114.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, A-549, HT-1080, Mouse kidney, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 34-290 of human IDS (NP_006114.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TDAL NVLL IIVD DLRP SLGC YGDK LVRS PNID QLAS HSLL FQNA FAQQ AVCA PSRV SFLT GRRP DTTR LYDF NSYW RVHA GNFS TIPQ YFKE NGYV TMSV GKVF HPGI SSNH TDDS PYSW SFPP YHPS SEKY ENTK TCRG PDGE LHAN LLCP VDVL DVPE GTLP DKQS TEQA IQLL EKMK TSAS PFFL AVGY HKPH IPFR YPKE FQKL YPLE NITL APDP EVPD GLPP VAYN PWMD IRQR EDVQ ALNI SVPY GPIP V
Gene ID:	3423
Uniprot:	P22304
Cellular Location:	Lysosome
Calculated MW:	34kDa/38kDa/61kDa
Observed MW:	76kDa

Synonyms:

IDS, MPS2, SIDS

Background:

This gene encodes a member of the sulfatase family of proteins. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. This enzyme is involved in the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

UniProt Protein Function:	IDS: Required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Defects in IDS are the cause of mucopolysaccharidosis type 2 (MPS2); also known as Hunter syndrome. MPS2 is an X-linked lysosomal storage disease characterized by intracellular accumulation of heparan sulfate and dermatan sulfate and their excretion in urine. Most children with MPS2 have a severe form with early somatic abnormalities including skeletal deformities, hepatosplenomegaly, and progressive cardiopulmonary deterioration. A prominent feature is neurological damage that presents as developmental delay and hyperactivity but progresses to mental retardation and dementia. They die before 15 years of age, usually as a result of obstructive airway disease or cardiac failure. In contrast, those with a mild form of MPS2 may survive into adulthood, with attenuated somatic complications and often without mental retardation. Belongs to the sulfatase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Hydrolase; EC 3.1.6.13; Glycan Metabolism - glycosaminoglycan degradation Chromosomal Location of Human Ortholog: Xq28 Cellular Component: lysosomal lumen Molecular Function:metal ion binding; iduronate-2-sulfatase activity Biological Process: chondroitin sulfate metabolic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; chondroitin sulfate catabolic process; pathogenesis Disease: Mucopolysaccharidosis, Type Ii
NCBI Summary:	The protein encoded by this gene belongs to the sulfatase family, is localized to the lysosome, and is involved in lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease, mucopolysaccharidosis type II, also known as Hunter syndrome. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Aug 2013]
UniProt Code:	P22304
NCBI GenInfo Identifier:	124174
NCBI Gene ID:	3423
NCBI Accession:	P22304.1
UniProt Secondary Accession:	P22304,Q14604, Q9BRM3, D3DWT4,
UniProt Related Accession:	P22304
Molecular Weight:	34,893 Da
NCBI Full Name:	Iduronate 2-sulfatase
NCBI Synonym Full Names:	iduronate 2-sulfatase
NCBI Official Symbol:	IDS
NCBI Official Synonym Symbols:	MPS2; SIDS
NCBI Protein Information:	iduronate 2-sulfatase; idursulfase; iduronate 2-sulfatase 14 kDa chain; iduronate 2-sulfatase 42 kDa chain; alpha-L-iduronate sulfate sulfatase
UniProt Protein Name:	Iduronate 2-sulfatase
UniProt Synonym Protein Names:	Alpha-L-iduronate sulfate sulfatase; Idursulfase
Protein Family:	Iduronate 2-sulfatase
UniProt Gene Name:	IDS
UniProt Entry Name:	IDS_HUMAN

	Western blot analysis of extracts of various cell lines, using IDS antibody (CAB1857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat brain using IDS Rabbit pAb (CAB1857) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using IDS Rabbit pAb (CAB1857) at dilution of 1:200 (40x lens).