Name: Anti-IDI1 Antibody (CAB13826)
Brand: Metabolism Antibodies 1
SKU: CAB13826
Availability: OutOfStock

Antibody Name:	Anti-IDI1 Antibody
Antibody SKU:	CAB13826
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 225-284 of human IDI1 (NP_004499.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	LO2, A431, HeLa, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 225-284 of human IDI1 (NP_004499.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IKSY CYVS KEEL KELL KKAA SGEI KITP WFKI IAAT FLFK WWDN LNHL NQFV DHEK IYRM
Gene ID:	3422
Uniprot:	Q13907
Cellular Location:	Peroxisome
Calculated MW:	26kDa/32kDa
Observed MW:	26kDa

Synonyms:

IDI1, IPP1, IPPI1

Background:

IDI1 encodes a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which are the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol. It has been shown in peroxisomal deficiency diseases such as Zellweger syndrome and neonatal adrenoleukodystrophy that there is reduction in IPP isomerase activity.

UniProt Protein Function:	IDI1: Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP). Belongs to the IPP isomerase type 1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Isomerase; EC 5.3.3.2; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis Chromosomal Location of Human Ortholog: 10p15.3 Cellular Component: mitochondrion; peroxisome; cytosol Molecular Function:hydrolase activity; metal ion binding; manganese ion binding; magnesium ion binding; isopentenyl-diphosphate delta-isomerase activity Biological Process: isoprenoid biosynthetic process; cholesterol biosynthetic process; dimethylallyl diphosphate biosynthetic process
NCBI Summary:	IDI1 encodes a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which are the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol. It has been shown in peroxisomal deficiency diseases such as Zellweger syndrome and neonatal adrenoleukodystrophy that there is reduction in IPP isomerase activity. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13907
NCBI GenInfo Identifier:	40018633
NCBI Gene ID:	3422
NCBI Accession:	NP_004499.2
UniProt Secondary Accession:	Q13907,Q32Q13, Q53GQ6, Q86U81, Q8WUX8, Q96IZ4, Q9BQ74 B4E155,
UniProt Related Accession:	Q13907
Molecular Weight:
NCBI Full Name:	isopentenyl-diphosphate Delta-isomerase 1
NCBI Synonym Full Names:	isopentenyl-diphosphate delta isomerase 1
NCBI Official Symbol:	IDI1
NCBI Official Synonym Symbols:	IPP1; IPPI1
NCBI Protein Information:	isopentenyl-diphosphate Delta-isomerase 1; IPP isomerase 1; isopentenyl pyrophosphate isomerase 1; isopentenyl diphosphate dimethylallyl diphosphate isomerase 1
UniProt Protein Name:	Isopentenyl-diphosphate Delta-isomerase 1
UniProt Synonym Protein Names:	Isopentenyl pyrophosphate isomerase 1
Protein Family:	Isopentenyl-diphosphate Delta-isomerase
UniProt Gene Name:	IDI1
UniProt Entry Name:	IDI1_HUMAN

Western blot analysis of extracts of various cell lines, using IDI1 antibody (CAB13826) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.

Antibodies

IDI1 Antibody (PACO09866)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)