Metabolism Antibodies 1

Anti-IDH3G Antibody (CAB13745)

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SKU:
CAB13745
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-IDH3G Antibody
Antibody SKU:CAB13745
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-170 of human IDH3G (NP_004126.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, A-431, HepG2, MCF-7, NIH/3T3, Mouse brain, Mouse heart, Mouse kidney, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 40-170 of human IDH3G (NP_004126.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FSEQ TIPP SAKY GGRH TVTM IPGD GIGP ELML HVKS VFRH ACVP VDFE EVHV SSNA DEED IRNA IMAI RRNR VALK GNIE TNHN LPPS HKSR NNIL RTSL DLYA NVIH CKSL PGVV TRHK DIDI LIVR ENT
Gene ID:3421
Uniprot:P51553
Cellular Location:Mitochondrion
Calculated MW:41kDa/42kDa
Observed MW:43kDa
Synonyms:IDH3G, H-IDHG
Background:Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined.
UniProt Protein Function:IDH3G: a enzyme that that catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (alpha-KG), requiring nicotinamide adenine dinucleotide (NAD) as a cofactor. The holoenzyme is a heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1. IDH3 is a mitochondrial enzyme that functions in the Krebs cycle, which generates substrates for cellular respiration. Alpha-KG can exit the mitochondria and enter other cellular compartments where it is a cofactor for the dioxygenases that hydroxylate the transcription factor HIF and trigger its degradation by VHL. Since HIF turns on oncogenic pathways, IDH3 may have tumor suppressor activity. Two isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - citrate (TCA) cycle; Oxidoreductase; EC 1.1.1.41; Mitochondrial

Chromosomal Location of Human Ortholog: Xq28

NCBI Summary:Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined. [provided by RefSeq, Jul 2008]
UniProt Code:P51553
NCBI GenInfo Identifier:1708404
NCBI Gene ID:3421
NCBI Accession:P51553.1
UniProt Secondary Accession:P51553,Q9BUU5, E9PDD5,
UniProt Related Accession:P51553
Molecular Weight:41,468 Da
NCBI Full Name:Isocitrate dehydrogenase
NCBI Synonym Full Names:isocitrate dehydrogenase 3 (NAD(+)) gamma
NCBI Official Symbol:IDH3G  
NCBI Official Synonym Symbols:H-IDHG  
NCBI Protein Information:isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
UniProt Protein Name:Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
UniProt Synonym Protein Names:Isocitric dehydrogenase subunit gamma; NAD(+)-specific ICDH subunit gamma
Protein Family:Isocitrate dehydrogenase
UniProt Gene Name:IDH3G  
UniProt Entry Name:IDH3G_HUMAN
Anti-IDH3G Antibody (CAB13745)Western blot analysis of extracts of various cell lines, using IDH3G antibody (CAB13745) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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