Metabolism Antibodies 1

Anti-IDH3B Antibody (CAB13742)

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SKU:
CAB13742
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-IDH3B Antibody
Antibody SKU:CAB13742
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 35-170 of human IDH3B (NP_008830.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:NCI-H460, HeLa, 293T, Mouse heart, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 35-170 of human IDH3B (NP_008830.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ASRS QAED VRVE GSFP VTML PGDG VGPE LMHA VKEV FKAA AVPV EFQE HHLS EVQN MASE EKLE QVLS SMKE NKVA IIGK IHTP MEYK GELA SYDM RLRR KLDL FANV VHVK SLPG YMTR HNNL DLVI IREQ TEGE
Gene ID:3420
Uniprot:O43837
Cellular Location:Mitochondrion
Calculated MW:25kDa/41kDa/42kDa
Observed MW:42KDa
Synonyms:IDH3B, RP46
Background:Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.
UniProt Protein Function:IDH3B: a enzyme that that catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (alpha-KG), requiring nicotinamide adenine dinucleotide (NAD) as a cofactor. The holoenzyme is a heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1. IDH3 is a mitochondrial enzyme that functions in the Krebs cycle, which generates substrates for cellular respiration. Alpha-KG can exit the mitochondria and enter other cellular compartments where it is a cofactor for the dioxygenases that hydroxylate the transcription factor HIF and trigger its degradation by VHL. Since HIF turns on oncogenic pathways, IDH3 has apparent tumor suppressor activity. Two isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Oxidoreductase; EC 1.1.1.41; Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: mitochondrial matrix; mitochondrion; nucleus

Molecular Function:electron carrier activity

Biological Process: tricarboxylic acid cycle

Disease: Retinitis Pigmentosa 46

NCBI Summary:Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:O43837
NCBI GenInfo Identifier:146345439
NCBI Gene ID:3420
NCBI Accession:O43837.2
UniProt Secondary Accession:O43837,O95106, Q5JXS8, Q9NQ06, Q9NQ07, Q9NUZ0, Q9UEX0 Q9UG99, B2RDR1, D3DVX2, D3DVX3,
UniProt Related Accession:O43837
Molecular Weight:25,555 Da
NCBI Full Name:Isocitrate dehydrogenase
NCBI Synonym Full Names:isocitrate dehydrogenase 3 (NAD(+)) beta
NCBI Official Symbol:IDH3B  
NCBI Official Synonym Symbols:RP46  
NCBI Protein Information:isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
UniProt Protein Name:Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
UniProt Synonym Protein Names:Isocitric dehydrogenase subunit beta; NAD(+)-specific ICDH subunit beta
Protein Family:Isocitrate dehydrogenase
UniProt Gene Name:IDH3B  
UniProt Entry Name:IDH3B_HUMAN
Anti-IDH3B Antibody (CAB13742)Western blot analysis of extracts of Rat heart, using IDH3B Rabbit pAb (CAB13742) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-IDH3B Antibody (CAB13742)Western blot analysis of extracts of various cell lines, using IDH3B Rabbit pAb (CAB13742) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-IDH3B Antibody (CAB13742)Immunofluorescence analysis of L929 cells using IDH3B Polyclonal Antibody (CAB13742) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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