Antibody Name:	Anti-IDH3A Antibody
Antibody SKU:	CAB14650
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 28-366 of human IDH3A (NP_005521.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Raji, NIH/3T3, Mouse brain, Mouse heart, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 28-366 of human IDH3A (NP_005521.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TGGV QTVT LIPG DGIG PEIS AAVM KIFD AAKA PIQW EERN VTAI QGPG GKWM IPSE AKES MDKN KMGL KGPL KTPI AAGH PSMN LLLR KTFD LYAN VRPC VSIE GYKT PYTD VNIV TIRE NTEG EYSG IEHV IVDG VVQS IKLI TEGA SKRI AEFA FEYA RNNH RSNV TAVH KANI MRMS DGLF LQKC REVA ESCK DIKF NEMY LDTV CLNM VQDP SQFD VLVM PNLY GDIL SDLC AGLI GGLG VTPS GNIG ANGV AIFE SVHG TAPD IAGK DMAN PTAL LLSA VMML RHMG LFDH AARI EAAC FATI KDGK SLTK DLGG NAKC SDFT EEIC RRVK DLD
Gene ID:	3419
Uniprot:	P50213
Cellular Location:	Mitochondrion
Calculated MW:	31kDa/40kDa
Observed MW:	40kDa

Synonyms:

IDH3A

Background:

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.

UniProt Protein Function:	IDH3A: a enzyme that that catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (alpha-KG), requiring nicotinamide adenine dinucleotide (NAD) as a cofactor. The holoenzyme is a heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1. IDH3 is a mitochondrial enzyme that functions in the Krebs cycle, which generates substrates for cellular respiration. Alpha-KG can exit the mitochondria and enter other cellular compartments where it is a cofactor for the dioxygenases that hydroxylate the transcription factor HIF and trigger its degradation by VHL. Since HIF turns on oncogenic pathways, IDH3 has apparent tumor suppressor activity. Two isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Mitochondrial; EC 1.1.1.41; Oxidoreductase; Carbohydrate Metabolism - citrate (TCA) cycle Chromosomal Location of Human Ortholog: 15q25.1-q25.2 Cellular Component: mitochondrial matrix; mitochondrion; nucleus Biological Process: tricarboxylic acid cycle
NCBI Summary:	Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq, Jul 2008]
UniProt Code:	P50213
NCBI GenInfo Identifier:	1708399
NCBI Gene ID:	3419
NCBI Accession:	P50213.1
UniProt Secondary Accession:	P50213,Q9H3X0, D3DW83,
UniProt Related Accession:	P50213
Molecular Weight:	31,381 Da
NCBI Full Name:	Isocitrate dehydrogenase
NCBI Synonym Full Names:	isocitrate dehydrogenase 3 (NAD(+)) alpha
NCBI Official Symbol:	IDH3A
NCBI Protein Information:	isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
UniProt Protein Name:	Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
UniProt Synonym Protein Names:	Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha
Protein Family:	Isocitrate dehydrogenase
UniProt Gene Name:	IDH3A
UniProt Entry Name:	IDH3A_HUMAN

	Western blot analysis of extracts of various cell lines, using IDH3A antibody (CAB14650) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat kidney using IDH3A antibody (CAB14650) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human thyroid cancer using IDH3A antibody (CAB14650) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using IDH3A antibody (CAB14650) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using IDH3A antibody (CAB14650) at dilution of 1:100 (40x lens).