Metabolism Antibodies 3
Anti-IDH1 Antibody (CAB5106)
- SKU:
- CAB5106
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-IDH1 Antibody |
| Antibody SKU: | CAB5106 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human IDH1 |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HepG2, A-549, MCF7, Mouse testis, Mouse brain, Mouse kidney, Rat liver |
| Immunogen: | A synthesized peptide derived from human IDH1 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 3417 |
| Uniprot: | O75874 |
| Cellular Location: | |
| Calculated MW: | 45kDa |
| Observed MW: | 45KDa |
| Synonyms: | HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD |
| Background: | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013] |
| UniProt Protein Function: | IDH1: an oxidoreductase that catalyzes the third step of the TCA cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH1 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. IDH1 is located in the cytosol and peroxisomes. Somatic mutations affecting arginine 132 (R132) are found in 80% of grade II?III gliomas and secondary glioblastomas in humans, and cause disease in a tissue-specific fashion. Only a single copy of the gene has been found to be mutated in tumours. Mutations of R132 to H, C, S, G, V, or L have been reported to be neomorphic, abolishing the conversion of isocitrate to alpha-KG. Instead, alpha-KG is converted to R(-)-2-hydroxyglutarate (2HG). Elevated levels of 2HG correlate with an elevated risk of malignant brain tumors. Neomorphic mutations in IDH1 and IDH2 convert alpha-KG to 2-hydroxyglutarate (2-HG) occur in a high percentage of patients with cytogenetically normal acute myeloid leukemia (AML). |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.1.1.42; Other Amino Acids Metabolism - glutathione; Oxidoreductase Chromosomal Location of Human Ortholog: 2q34 Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; peroxisomal matrix; peroxisome Molecular Function:(R)-2-hydroxyglutarate dehydrogenase activity; isocitrate dehydrogenase (NADP+) activity; magnesium ion binding; protein homodimerization activity; receptor binding Biological Process: 2-oxoglutarate metabolic process; isocitrate metabolic process; NADPH regeneration Disease: Glioma Susceptibility 1 |
| NCBI Summary: | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013] |
| UniProt Code: | O75874 |
| NCBI GenInfo Identifier: | 21903432 |
| NCBI Gene ID: | 3417 |
| NCBI Accession: | O75874.2 |
| UniProt Secondary Accession: | O75874,Q567U4, Q6FHQ6, Q7Z3V0, Q93090, Q9NTJ9, Q9UKW8 |
| UniProt Related Accession: | O75874 |
| Molecular Weight: | 46,659 Da |
| NCBI Full Name: | Isocitrate dehydrogenase |
| NCBI Synonym Full Names: | isocitrate dehydrogenase (NADP(+)) 1, cytosolic |
| NCBI Official Symbol: | IDH1Â Â |
| NCBI Official Synonym Symbols: | IDH; IDP; IDCD; IDPC; PICD; HEL-216; HEL-S-26Â Â |
| NCBI Protein Information: | isocitrate dehydrogenase [NADP] cytoplasmic |
| UniProt Protein Name: | Isocitrate dehydrogenase [NADP] cytoplasmic |
| UniProt Synonym Protein Names: | Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase |
| Protein Family: | Isocitrate dehydrogenase |
| UniProt Gene Name: | IDH1Â Â |
 | Western blot - IDH1 Rabbit mAb (CAB5106) |
View AllClose
Related Products
![Anti-IDH1 Antibody (CAB13245)[KO Validated]](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/590x590/products/40564/45309/anti-idh1-antibody-cab13245ko-validated__44948__41417.1706521924.jpg?c=1 "Anti-IDH1 Antibody (CAB13245)[KO Validated]")
![Anti-IDH1 Antibody (CAB18023)[KO Validated]](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/590x590/products/55168/59911/anti-idh1-antibody-cab18023ko-validated__21082__83222.1706529185.jpg?c=1 "Anti-IDH1 Antibody (CAB18023)[KO Validated]")
IDH1 Polyclonal Antibody
IDH1 Polyclonal AntibodyThe product page for this antibody is being prepared. If you have a question please don't hesitate to get in touch!
