Antibody Name:	Anti-IDE Antibody [KO Validated]
Antibody SKU:	CAB1630
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human IDE (NP_004960.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	DU145, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human IDE (NP_004960.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MRYR LAWL LHPA LPST FRSV LGAR LPPP ERLC GFQK KTYS KMNN PAIK RIGN HITK SPED KREY RGLE LANG IKVL LISD PTTD KSSA ALDV HIGS LSDP PNIA GLSH FCEH MLFL GTKK YPKE NEYS QFLS EHAG SSNA FTSG EHTN YYFD VSHE HLEG ALDR FAQF FLCP LFDE SCKD REVN AVDS EHEK NVMN DAWR LFQL EKAT GNPK HPFS KFGT GNKY TLET RPNQ EGID VRQE LLKF HSAY YS
Gene ID:	3416
Uniprot:	P14735
Cellular Location:	Cell membrane, Cytoplasm, Secreted
Calculated MW:	54kDa/117kDa
Observed MW:	118kDa

Synonyms:

IDE, INSULYSIN

Background:

This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.

UniProt Protein Function:	IDE: Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. Belongs to the peptidase M16 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell surface; EC 3.4.24.56; Nuclear receptor co-regulator; Protease Chromosomal Location of Human Ortholog: 10q23.33 Cellular Component: cell surface; cytoplasm; cytosol; extracellular space; mitochondrion; nucleus; peroxisome Molecular Function:ATP binding; glycoprotein binding; insulin binding; metalloendopeptidase activity; peptide binding; protein binding; protein homodimerization activity; receptor binding; ubiquitin binding; zinc ion binding Biological Process: beta-amyloid metabolic process; determination of adult life span; positive regulation of protein oligomerization; protein homooligomerization; proteolysis; proteolysis involved in cellular protein catabolic process
NCBI Summary:	This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009]
UniProt Code:	P14735
NCBI GenInfo Identifier:	215274252
NCBI Gene ID:	3416
NCBI Accession:	P14735.4
UniProt Secondary Accession:	P14735,Q5T5N2, B2R721, B7ZAU2, D3DR35,
UniProt Related Accession:	P14735
Molecular Weight:	118kDa
NCBI Full Name:	Insulin-degrading enzyme
NCBI Synonym Full Names:	insulin degrading enzyme
NCBI Official Symbol:	IDE
NCBI Official Synonym Symbols:	INSULYSIN
NCBI Protein Information:	insulin-degrading enzyme
UniProt Protein Name:	Insulin-degrading enzyme
UniProt Synonym Protein Names:	Abeta-degrading protease; Insulin protease; Insulinase; Insulysin
Protein Family:	IgM protease
UniProt Gene Name:	IDE

	Western blot analysis of extracts of various cell lines, using IDE antibody (CAB1630) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
	Western blot analysis of extracts from normal (control) and IDE knockout (KO) HeLa cells, using IDE antibody (CAB1630) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of A549 cells using IDE antibody (CAB1630). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-IDE Antibody (CAB11190)	Human IDE / Insulin-degrading enzyme ELISA Kit
IDE Antibody (PACO09862)	Human IDE (Insulin Degrading Enzyme) CLIA Kit (HUES00310)
	Human IDE (Insulin Degrading Enzyme) ELISA Kit (HUES01641)
	Human Insulin-degrading enzyme (IDE) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)