Metabolism Antibodies 1

Anti-ICT1 Antibody (CAB11590)

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SKU:
CAB11590
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-ICT1 Antibody
Antibody SKU:CAB11590
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-206 of human ICT1 (NP_001536.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Raji, HT-29, 293T, B cells, SKOV3, Mouse lung, Mouse kidney, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-206 of human ICT1 (NP_001536.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LHKQ KDGT EFKS IYSL DKLY PESQ GSDT AWRV PNGA KQAD SDIP LDRL TISY CRSS GPGG QNVN KVNS KAEV RFHL ATAE WIAE PVRQ KIAI THKN KINR LGEL ILTS ESSR YQFR NLAD CLQK IRDM ITEA SQTP KEPT KEDV KLHR IRIE NMNR ERLR QKRI HSAV KTSR RVDM D
Gene ID:3396
Uniprot:Q14197
Cellular Location:Mitochondrion
Calculated MW:23kDa
Observed MW:24kDa
Synonyms:MRPL58, DS-1, DS1, ICT1, MRP-L58
Background:The protein encoded by this gene is a peptidyl-tRNA hydrolase and a vital component of the large mitochondrial ribosome. The encoded protein serves as a ribosome release factor for this ribosome, which translates mitochondrial genes. This protein may be responsible for degrading prematurely-terminated polypeptides and for reusing stalled ribosomes. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:ICT1: Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes. Belongs to the prokaryotic/mitochondrial release factor family. ICT1 subfamily.
UniProt Protein Details:

Protein type:EC 3.1.1.29; Mitochondrial

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: mitochondrial inner membrane; mitochondrial large ribosomal subunit; mitochondrial matrix; mitochondrion

Molecular Function:aminoacyl-tRNA hydrolase activity; translation release factor activity, codon nonspecific

NCBI Summary:The protein encoded by this gene is a peptidyl-tRNA hydrolase and a vital component of the large mitochondrial ribosome. The encoded protein serves as a ribosome release factor for this ribosome, which translates mitochondrial genes. This protein may be responsible for degrading prematurely-terminated polypeptides and for reusing stalled ribosomes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]
UniProt Code:Q14197
NCBI GenInfo Identifier:6015033
NCBI Gene ID:3396
NCBI Accession:Q14197.1
UniProt Secondary Accession:Q14197,Q53HM7, Q53Y11, B2RAD1,
UniProt Related Accession:Q14197
Molecular Weight:24kDa
NCBI Full Name:Peptidyl-tRNA hydrolase ICT1, mitochondrial
NCBI Synonym Full Names:mitochondrial ribosomal protein L58
NCBI Official Symbol:MRPL58  
NCBI Official Synonym Symbols:DS1; DS-1; ICT1; MRP-L58  
NCBI Protein Information:peptidyl-tRNA hydrolase ICT1, mitochondrial
UniProt Protein Name:Peptidyl-tRNA hydrolase ICT1, mitochondrial
UniProt Synonym Protein Names:39S ribosomal protein L58, mitochondrial
Protein Family:1-acylglycerol-3-phosphate O-acyltransferase
UniProt Gene Name:MRPL58  
Anti-ICT1 Antibody (CAB11590)Western blot analysis of extracts of various cell lines, using ICT1 antibody (CAB11590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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