Cell Biology Antibodies 11

Anti-ICK Antibody (CAB7783)

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SKU:
CAB7783
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-ICK Antibody
Antibody SKU:CAB7783
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ICK (NP_057597.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, SW620, Mouse liver, Mouse lung, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ICK (NP_057597.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MNRY TTIR QLGD GTYG SVLL GRSI ESGE LIAI KKMK RKFY SWEE CMNL REVK SLKK LNHA NVVK LKEV IREN DHLY FIFE YMKE NLYQ LIKE RNKL FPES AIRN IMYQ ILQG LAFI HKHG FFHR DLKP ENLL CMGP ELVK IADF GLAR EIRS KPPY TDYV STRW YRAP EVLL RSTN YSSP IDVW AVGC IMAE VYTL RPLF PGAS EIDT IFKI CQVL GTPK KTDW PEGY QLSS AMNF RWPQ CVPN NLKT LIPN ASSE AVQL LRDM LQWD PK
Gene ID:22858
Uniprot:Q9UPZ9
Cellular Location:Cell projection, Cytoplasm, Nucleus, cilium, cilium basal body, cytoskeleton, cytosol
Calculated MW:34kDa/71kDa
Observed MW:71kDa
Synonyms:ICK, ECO, LCK2, MRK
Background:Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified.
UniProt Protein Function:Required for ciliogenesis (PubMed:24797473). Phosphorylates KIF3A. Involved in the control of ciliary length (PubMed:24853502). Regulates the ciliary localization of SHH pathway components as well as the localization of IFT components at ciliary tips. May play a key role in the development of multiple organ systems and particularly in cardiac development. Regulates intraflagellar transport (IFT) speed and negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner and this regulation requires its kinase activity.
NCBI Summary:Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:Q9UPZ9
NCBI GenInfo Identifier:7662388
NCBI Gene ID:22858
NCBI Accession:NP_055735.1
UniProt Secondary Accession:Q9UPZ9,O75985, Q5THL2, Q8IYH8, Q9BX17, Q9NYX3, A7MD41
UniProt Related Accession:Q9UPZ9
Molecular Weight:71 kDa
NCBI Full Name:serine/threonine-protein kinase ICK
NCBI Synonym Full Names:intestinal cell kinase
NCBI Official Symbol:ICK  
NCBI Official Synonym Symbols:ECO; MRK; LCK2  
NCBI Protein Information:serine/threonine-protein kinase ICK
UniProt Protein Name:Serine/threonine-protein kinase ICK
UniProt Synonym Protein Names:Intestinal cell kinase; hICK; Laryngeal cancer kinase 2; LCK2; MAK-related kinase; MRK
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:ICK  
Anti-ICK Antibody (CAB7783)Western blot analysis of extracts of various cell lines, using ICK antibody (CAB7783) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-ICK Antibody (CAB7783)Immunofluorescence analysis of U2OS cells using ICK antibody (CAB7783) at dilution of 1:100. Blue: DAPI for nuclear staining.
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