Cell Biology Antibodies 5

Anti-IBTK Antibody (CAB14866)

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SKU:
CAB14866
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-IBTK Antibody
Antibody SKU:CAB14866
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 900-1200 of human IBTK (NP_056340.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:Jurkat, HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 900-1200 of human IBTK (NP_056340.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GLNM AALL EARS LDVL SDGV LKDL SEFY RKMI PAMD RRVI TPYQ DGPD ISYL EVED GDIF LKEE INME QNHS ETMF KKAK TKAK KKPR KRSD SSGG YNLS DIIQ SPSS TGLL KSGK TNSV ESLP ELLT SDSE GSYA GVGS PRDL QSPD FTTG FHSD KIEA KVKP YVNG TSPV YSRE DLKP WEKS PILK ISAP QPIP SNRI DTTS SASW VAGS FSPV SPPV VDLR TIME IEES RQKC GATP KSHL GKTV SHGV KLSQ KQRK MIAL TTKE NNSG MNSM ETVL FTPS KAPK PVNA WASS LHSV S
Gene ID:25998
Uniprot:Q9P2D0
Cellular Location:Cytoplasm, Membrane, Nucleus, Peripheral membrane protein
Calculated MW:26kDa/133kDa/150kDa
Observed MW:150kDa
Synonyms:IBTK, BTBD26, BTKI
Background:Bruton tyrosine kinase (BTK) is a protein tyrosine kinase that is expressed in B cells, macrophages, and neutrophils. The protein encoded by this gene binds to BTK and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription. This gene has a pseudogene on chromosome 18. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
UniProt Protein Function:IBTK: Acts as an inhibitor of BTK tyrosine kinase activity, thereby playing a role in B-cell development. Down-regulates BTK kinase activity, leading to interference with BTK-mediated calcium mobilization and NF-kappa-B-driven transcription. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 6q14.1

Cellular Component: cytoplasm; nucleoplasm

Molecular Function:protein kinase binding; protein tyrosine kinase inhibitor activity

Biological Process: negative regulation of protein amino acid phosphorylation; release of sequestered calcium ion into cytosol

NCBI Summary:Bruton tyrosine kinase (BTK) is a protein tyrosine kinase that is expressed in B cells, macrophages, and neutrophils. The protein encoded by this gene binds to BTK and downregulates BTK's kinase activity. In addition, the encoded protein disrupts BTK-mediated calcium mobilization and negatively regulates the activation of nuclear factor-kappa-B-driven transcription. This gene has a pseudogene on chromosome 18. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]
UniProt Code:Q9P2D0
NCBI GenInfo Identifier:134034141
NCBI Gene ID:25998
NCBI Accession:Q9P2D0.3
UniProt Secondary Accession:Q9P2D0,Q2QKU2, Q2QKU3, Q2QKU4, Q5TFD7, Q5TFD9, Q8IUQ9 Q8IUY7, Q8TAI4, Q9HBI8, Q9Y3T8,
UniProt Related Accession:Q9P2D0
Molecular Weight:26,314 Da
NCBI Full Name:Inhibitor of Bruton tyrosine kinase
NCBI Synonym Full Names:inhibitor of Bruton tyrosine kinase
NCBI Official Symbol:IBTK  
NCBI Official Synonym Symbols:BTKI; BTBD26  
NCBI Protein Information:inhibitor of Bruton tyrosine kinase
UniProt Protein Name:Inhibitor of Bruton tyrosine kinase
Protein Family:Inhibitor of Bruton tyrosine kinase
UniProt Gene Name:IBTK  
UniProt Entry Name:IBTK_HUMAN
Anti-IBTK Antibody (CAB14866)Western blot analysis of extracts of various cell lines, using IBTK antibody (CAB14866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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