Cell Biology Antibodies 3
Anti-Iba1 Antibody (CAB12391)
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- SKU:
- CAB12391
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Iba1 Antibody |
| Antibody SKU: | CAB12391 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human Iba1 (NP_001614.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | THP-1, Mouse spleen, Rat testis, Rat spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human Iba1 (NP_001614.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSQT RDLQ GGKA FGLL KAQQ EERL DEIN KQFL DDPK YSSD EDLP SKLE GFKE KYME FDLN GNGD IDIM SLKR MLEK LGVP KTHL ELKK LIGE VSSG SGET FSYP DFLR MMLG KRSA ILKM ILMY EEKA REKE KPTG PPAK KAIS ELP |
| Gene ID: | 199 |
| Uniprot: | P55008 |
| Cellular Location: | Cell projection, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cytoskeleton, ruffle membrane |
| Calculated MW: | 10kDa/14kDa/16kDa |
| Observed MW: | 15KDa |
| Synonyms: | AIF1, AIF-1, IBA1, IRT-1, IRT1, Iba1 |
| Background: | This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. |
| UniProt Protein Function: | AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 6p21.3 Cellular Component: perinuclear region of cytoplasm; lamellipodium; cytoplasm; actin filament; perikaryon; nucleus; cytosol; phagocytic cup Molecular Function:actin filament binding; calcium ion binding Biological Process: actin filament bundle formation; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; microglial cell activation; Rac protein signal transduction; cellular response to hormone stimulus; actin filament polymerization; positive regulation of muscle hyperplasia; cellular response to extracellular stimulus; positive regulation of T cell proliferation; response to axon injury; positive regulation of protein amino acid phosphorylation; phagocytosis, engulfment; response to electrical stimulus; inflammatory response; negative regulation of apoptosis |
| NCBI Summary: | This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016] |
| UniProt Code: | P55008 |
| NCBI GenInfo Identifier: | 1703217 |
| NCBI Gene ID: | 199 |
| NCBI Accession: | P55008.1 |
| UniProt Related Accession: | P55008 |
| Molecular Weight: | |
| NCBI Full Name: | Allograft inflammatory factor 1 |
| NCBI Synonym Full Names: | allograft inflammatory factor 1 |
| NCBI Official Symbol: | AIF1 |
| NCBI Official Synonym Symbols: | IBA1; IRT1; AIF-1; IRT-1 |
| NCBI Protein Information: | allograft inflammatory factor 1 |
| UniProt Protein Name: | Allograft inflammatory factor 1 |
| UniProt Synonym Protein Names: | Ionized calcium-binding adapter molecule 1; Protein G1 |
| Protein Family: | Putative apoptosis-inducing factor |
| UniProt Gene Name: | AIF1 |
| UniProt Entry Name: | AIF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Iba1 Rabbit pAb (CAB12391) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s. |
 | Immunohistochemistry of paraffin-embedded rat bone marrow using Iba1 antibody (CAB12391) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using Iba1 antibody (CAB12391) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of rat brain using Iba1 Rabbit pAb (CAB12391) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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