Cell Biology Antibodies 12
Anti-HYLS1 Antibody (CAB8285)
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- SKU:
- CAB8285
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IF
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | HYLS1 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8285 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 70-299 of human HYLS1 (NP_659451.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | MCF7 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 70-299 of human HYLS1 (NP_659451.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YPHV ESNV PSET VSEA SQRL RKPV MKRK VLRR KPDG EVLV TDES IISE SESG TEND QDLW DLRQ RLMN VQFQ EDKE SSFD VSQK FNLP HEYQ GISQ DQLI CSLQ REGM GSPA YEQD LIVA SRPK SFIL PKLD QLSR NRGK TDRV ARYF EYKR DWDS IRLP GEDH RKEL RWGV REQM LCRA EPQS KPQH IYVP NNYL VPTE KKRS ALRW GVRC DLAN GVIP RKLP FPLS PS |
| Gene ID: | 219844 |
| Uniprot: | Q96M11 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 34kDa |
| Observed MW: | 37kDa |
| Synonyms: | HYLS1, HLS |
| Background: | This gene encodes a protein localized to the cytoplasm. Mutations in this gene are associated with hydrolethalus syndrome. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| UniProt Code: | Q96M11 |
| NCBI GenInfo Identifier: | 198278446 |
| NCBI Gene ID: | |
| NCBI Accession: | NP_001128265.1 |
| Molecular Weight: | 34kDa |
| NCBI Full Name: | hydrolethalus syndrome protein 1 |
| UniProt Protein Name: | Hydrolethalus syndrome protein 1 |
| Protein Family: | Hydrolethalus syndrome protein |
| UniProt Gene Name: | HYLS1 |
| UniProt Entry Name: | HYLS1_HUMAN |
 | Western blot analysis of extracts of MCF-7 cells, using HYLS1 antibody (CAB8285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using HYLS1 antibody (CAB8285) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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