Cell Biology Antibodies 10
Anti-HYAL2 Antibody (CAB6624)
- SKU:
- CAB6624
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-HYAL2 Antibody |
| Antibody SKU: | CAB6624 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 367-473 of human HYAL2 (NP_149348.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Mouse liver, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 367-473 of human HYAL2 (NP_149348.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RAQC HGHG RCVR RNPS ASTF LHLS TNSF RLVP GHAP GEPQ LRPV GELS WADI DHLQ THFR CQCY LGWS GEQC QWDH RQAA GGAS EAWA GSHL TSLL ALAA LAFT WTL |
| Gene ID: | 8692 |
| Uniprot: | Q12891 |
| Cellular Location: | Cell membrane, GPI-anchor, Lipid-anchor |
| Calculated MW: | 53kDa |
| Observed MW: | 54kDa |
| Synonyms: | HYAL2, LUCA2 |
| Background: | This gene encodes a weak acid-active hyaluronidase. The encoded protein is similar in structure to other more active hyaluronidases. Hyaluronidases degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan and fragments of hyaluronan are thought to be involved in cell proliferation, migration and differentiation. Although it was previously thought to be a lysosomal hyaluronidase that is active at a pH below 4, the encoded protein is likely a GPI-anchored cell surface protein. This hyaluronidase serves as a receptor for the oncogenic virus Jaagsiekte sheep retrovirus. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes two alternatively spliced transcript variants which differ only in the 5' UTR. |
| UniProt Protein Function: | HYAL2: Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R. Belongs to the glycosyl hydrolase 56 family. |
| UniProt Protein Details: | Protein type:EC 3.2.1.35; Glycan Metabolism - glycosaminoglycan degradation; Membrane protein, GPI anchor; Hydrolase Chromosomal Location of Human Ortholog: 3p21.3 Cellular Component: microvillus; cell surface; endoplasmic reticulum; lysosome; cytoplasmic membrane-bound vesicle; cytosol; lipid raft; Golgi membrane; anchored to external side of plasma membrane; anchored to plasma membrane; endocytic vesicle; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; plasma membrane; cytoplasmic vesicle Molecular Function:viral receptor activity; receptor signaling protein tyrosine kinase inhibitor activity; protein binding; enzyme binding; transforming growth factor beta binding; hyaluronic acid binding; hyaluronoglucuronidase activity; receptor tyrosine kinase binding; hyalurononglucosaminidase activity Biological Process: negative regulation of MAP kinase activity; entry of virus into host cell; negative regulation of protein kinase B signaling cascade; glycosaminoglycan metabolic process; response to virus; multicellular organismal aging; pathogenesis; hyaluronan catabolic process; response to antibiotic; response to reactive oxygen species; glycosaminoglycan catabolic process; viral envelope fusion with host membrane; monocyte activation; positive regulation of protein import into nucleus; skeletal morphogenesis; cartilage development; carbohydrate metabolic process; hemopoietic progenitor cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; kidney development; defense response to virus; hyaluronan metabolic process; positive regulation of inflammatory response; transformation of host cell by virus |
| NCBI Summary: | This gene encodes a weak acid-active hyaluronidase. The encoded protein is similar in structure to other more active hyaluronidases. Hyaluronidases degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan and fragments of hyaluronan are thought to be involved in cell proliferation, migration and differentiation. Although it was previously thought to be a lysosomal hyaluronidase that is active at a pH below 4, the encoded protein is likely a GPI-anchored cell surface protein. This hyaluronidase serves as a receptor for the oncogenic virus Jaagsiekte sheep retrovirus. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes two alternatively spliced transcript variants which differ only in the 5' UTR.[provided by RefSeq, Mar 2010] |
| UniProt Code: | Q12891 |
| NCBI GenInfo Identifier: | 311033483 |
| NCBI Gene ID: | 8692 |
| NCBI Accession: | Q12891.4 |
| UniProt Secondary Accession: | Q12891,O15177, Q9BW29, B3KRZ2, |
| UniProt Related Accession: | Q12891 |
| Molecular Weight: | 53,860 Da |
| NCBI Full Name: | Hyaluronidase-2 |
| NCBI Synonym Full Names: | hyaluronoglucosaminidase 2 |
| NCBI Official Symbol: | HYAL2 |
| NCBI Official Synonym Symbols: | LUCA2 |
| NCBI Protein Information: | hyaluronidase-2; hyal-2; PH20 homolog; PH-20 homolog; hyaluronidase 2; lysosomal hyaluronidase; lung carcinoma protein 2; hyaluronoglucosaminidase-2 |
| UniProt Protein Name: | Hyaluronidase-2 |
| UniProt Synonym Protein Names: | Hyaluronoglucosaminidase-2; Lung carcinoma protein 2; LuCa-2 |
| UniProt Gene Name: | HYAL2 |
| UniProt Entry Name: | HYAL2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HYAL2 antibody (CAB6624) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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