Product Name:	Anti-Human TCR Vα24-Jα18 (iNKT cell) In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0136
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	6B11
Protein:	TCR Vα24-Jα18
Product Type:	Monoclonal Antibody
Synonyms:	TCR Vα24-Jα18, TCR Vα24-JαQ, invariant NKT cell, iNKT
Isotype:	Mouse IgG1 κ
Reactivity:	Human
Applications:	CyTOF®, FC
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0136	Anti-Human TCR Vα24-Jα18 (iNKT cell) In Vivo Antibody - Low Endotoxin	Low
IVMB0137	Anti-Human TCR Vα24-Jα18 (iNKT cell) In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	CyTOF®, FC
Recommended Usage:	FC The suggested concentration for this TCR Vα24-Jα18 antibody for staining cells in flow cytometry is ≤ 1.0 µg per 10⁶ in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Reactivity:	Human

Host Species:	Mouse
Specificity:	Clone 6B11 recognizes the invariant CDR3 region of the human Vα24-Jα18 TCR.
Antigen Distribution:	The Vα24-Jα18 TCR is expressed on iNKT cells.
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

TCR Vα24-Jα18 antibody, 6B11, recognizes the CDR3 region of the V alpha 24 and J alpha 18 TCR alpha locus (Vα24-Jα18). The Vα24-Jα18 semi-invariant TCR is expressed on a highly conserved T cell subset, named invariant natural killer T (iNKT cells), which also express NK cell-associated molecules. The Vα24-Jα18 TCR recognizes lipid antigens, such as α-galactosylceramide (α-GalCer), presented by the MHC class I-like molecule CD1d1. Following activation, iNKT cells rapidly proliferate and produce large amounts of IL-4, IFNγ, and cytolytic mediators2,3. iNKT cells have both inflammatory and immunoregulatory functions and are implicated in anti-tumor immunity, protection against infections, suppression of autoimmunity, and the development of inflammatory diseases4.