Anti-Human IgM µ-Chain Specific In Vivo Antibody - Low Endotoxin
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- SKU:
- IVMB0367
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- HB57
- Protein:
- IgM µ-Chain Specific
- Isotype:
- IgG1
- Reactivity:
- Human
- Endotoxin Level:
- Low Endotoxin
- Host Species:
- Mouse
- Applications:
- ELISA
- Applications:
- FC
- Applications:
- In Vivo
Description
| Product Name: | Anti-Human IgM µ-Chain Specific In Vivo Antibody - Low Endotoxin |
| Product Code: | IVMB0367 |
| Size: | 0.25 mg |
| Clone: | HB57 |
| Protein: | IgM µ-Chain Specific |
| Product Type: | Monoclonal Antibody |
| Isotype: | IgG1 |
| Reactivity: | Human |
| Applications: | ELISA, FC, In Vivo |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Purity: | ≥95% monomer by analytical SEC ⋅ >95% by SDS Page |
| Product Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Applications: | ELISA, FC, In Vivo |
| Reactivity: | Human |
| Cross Reactivity: | Cross-reactivity by ELISA against human myeloma proteins: Human IgG1: 100% Human IgG2: 100% Human IgG3: 100% Human IgG4: 100% Human IgM: <0.2% Human IgA: <0.1% Human IgE: <0.1% There is no detectable binding to bovine, goat, horse sheep IgG. NOTE: This Anti-Human IgG, Fc Fragment Specific (Clone HB57) when conjugated to HRP is validated for use in Enzyme Immunoassay for the detection of Human IgG, µ-Chain Specific (Part No.: I-1201) |
| Host Species: | Mouse |
| Specificity: | Anti-Human IgM (µ chain specific) monoclonal antibody reacts with human IgM through an epitope on the heavy chain. This antibody is non-reactive with IgG, IgA or light chains. Clone HB57 produces an antibody with one of the highest affinities available (Ka = 5.34 x 108 M-1).1 |
| Antigen Distribution: | Surface IgM is expressed on B-lymphocytes. |
| Concentration: | ≥ 5.0 mg/ml |
| Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
| Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. |
| Purity: | ≥95% monomer by analytical SEC ⋅ >95% by SDS Page |
| Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
| Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
| Protein: | IgM µ-Chain Specific |
| Shipping: | 2-8°C |
| PubMed: | IgM µ-Chain Specific |
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