Product Name:	Anti-Human CD62L In Vivo Antibody - Low Endotoxin
Product Code:	IVMB0121
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	DREG56
Protein:	CD62L
Product Type:	Monoclonal Antibody
Synonyms:	TQ1, LAM1, LEU8, LNHR, LSEL, CD62L, LYAM1, PLNHR, LECAM1, L-selectin
Isotype:	Mouse IgG1
Reactivity:	Human
Immunogen:	Concentrated supernatant from PMA-activated human peripheral blood leukocytes
Applications:	B, FC, IF, IHC FF, PhenoCycler®, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0121	Anti-Human CD62L In Vivo Antibody - Low Endotoxin	Low
IVMB0122	Anti-Human CD62L In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	B, CODEX®, FC, IF, IHC FF, WB
Recommended Usage:	FC The suggested concentration for this DREG56 antibody for staining cells in flow cytometry is ≤ 0.5 µg per 10⁶ in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this DREG56 antibody for use in western blotting is 1-10 µg/ml.
Reactivity:	Human

Host Species:	Mouse
Specificity:	Clone DREG56 recognizes an epitope on human CD62L.
Antigen Distribution:	CD62L is expressed on most circulating leukocytes, including neutrophils, monocytes, T, B and NK cells, and human embryo trophoblasts.
Immunogen:	Concentrated supernatant from PMA-activated human peripheral blood leukocytes
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	< 1.0 EU/mg as determined by the LAL method
Purity:	≥95% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

CD62L antibody, clone DREG56, recognizes CD62L, also known as L-selectin or LECAM-1, a 74-95 kDa type I transmembrane glycoprotein and cell adhesion molecule expressed on most circulating leukocytes1. Ligands for CD62L include CD34, GlyCAM-1, and MAdCAM-1, which require sialyation, fucosylation, and carbohydrate sulfation for recognition2,3. Ligand binding mediates tethering and rolling of leukocytes, facilitating entry into secondary lymphoid organs, including lymph nodes and Peyer’s patches, via high endothelial venules (HEVs)1. CD62L is also expressed on human embryo trophoblasts and has been proposed to play a role in human embryo implantation4.

Technical Datasheet:	View
Protein:	CD62L
Function:	Leukocyte homing, leukocyte tethering, rolling
Ligand/Receptor:	CD34, GlyCAM, MAdCAM-1
Research Area:	Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity