Anti-Human CD4 [OKT-4] In Vivo Antibody - Low Endotoxin
- SKU:
- IVMB0012
- Product Type:
- In Vivo Monoclonal Antibody
- Clone:
- OKT-4
- Protein:
- CD4
- Isotype:
- Mouse IgG2b kappa
- Reactivity:
- Human
- Synonyms:
- CD4
- Synonyms:
- T4
- Synonyms:
- CD4 antigen (p55)
- Synonyms:
- CD4 antigen p55
- Synonyms:
- CD4 receptor
- Synonyms:
- T-cell surface antigen T4/Leu-3
- Synonyms:
- T-cell surface glycoprotein CD4
- Endotoxin Level:
- Low Endotoxin
- Host Species:
- Mouse
- Applications:
- CODEX
- Applications:
- ELISA
- Applications:
- FC
- Applications:
- ICC
- Applications:
- IF Staining
- Applications:
- IHC FF
- Applications:
- IHC FFPE
- Applications:
- IP
- Applications:
- Neutralizing
- Applications:
- WB
Description
system_update_altTechnical Datasheet
Product Name: | Anti-Human CD4 [OKT-4] In Vivo Antibody - Low Endotoxin |
Product Code: | IVMB0012 |
Size: | 1mg, 5mg, 25mg, 50mg, 100mg |
Clone: | OKT-4 |
Protein: | CD4 |
Product Type: | Monoclonal Antibody |
Synonyms: | CD4, T4, CD4 antigen (p55), CD4 antigen p55, CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4 |
Isotype: | Mouse IgG2b κ |
Reactivity: | Human |
Immunogen: | Human peripheral T cells |
Applications: | ELISA, FC, ICC, IF Staining, IHC FF, IHC FFPE, IP, N, PhenoCycler®, WB |
Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
Applications: | CODEX®, ELISA, FC, ICC, IF Staining, IHC FF, IHC FFPE, IP, N, WB |
Recommended Usage: | ELISA FC WB |
Reactivity: | Human |
Host Species: | Mouse |
Specificity: | Clone OKT4 recognizes Human CD4. This clone recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not interfere with each other. |
Antigen Distribution: | CD4 is expressed on most thymocytes, a subset of T cells, and monocytes/macrophages, and may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. |
Immunogen: | Human peripheral T cells |
Concentration: | ≥ 5.0 mg/ml |
Endotoxin Level: | < 1.0 EU/mg as determined by the LAL method |
Purity: | ≥95% monomer by analytical SEC >95% by SDS Page |
Formulation: | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. |
Preparation: | Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
Storage and Handling: | Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
CD4 is a 55 kD single-chain type I transmembrane glycoprotein and a member of the Ig superfamily. It is involved in the recognition of MHC class II molecules. Furthermore, CD4 is the primary receptor for HIV. CD4 initiates the early phase of T-cell activation via its association with the T-cell receptor complex and protein tyrosine kinase, Lck. In addition, CD4 functions as a mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system.
Technical Datasheet: | View |
Protein: | CD4 |
Research Area: | Immunology |
Mouse IgG2b Isotype Control | |
---|---|
Clone | MPC-11 |
Isotype | Mouse IgG2b kappa |
Endotoxin Level | Low Endotoxin |
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Shane Costigan
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Abdul Khadim
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