Anti-Human CD18 (ITGB2) In Vivo Antibody - Ultra Low Endotoxin

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SKU:
IVMB0251
Product Type:
Hybridoma Monoclonal Antibody
Clone:
IB4
Protein:
CD18
Isotype:
IgG2a k
Reactivity:
Human
Synonyms:
Integrin beta-2
Synonyms:
integrin beta chain
Synonyms:
beta 2
Synonyms:
ITGB2
Research Area:
Cell Adhesion
Research Area:
Cell Biology
Research Area:
Immunology
Research Area:
Signal Transduction
Endotoxin Level:
Ultra Low Endotoxin
Host Species:
Mouse
Applications:
FA
Applications:
FC
Applications:
IF Microscopy
Applications:
In Vivo

Description

Product Name:Anti-Human CD18 (ITGB2) In Vivo Antibody - Ultra Low Endotoxin
Product Code:IVMB0251
Size:1 mg, 5 mg, 25 mg, 50 mg, 100 mg
Clone:IB4
Protein:CD18
Product Type:Hybridoma Monoclonal Antibody
Synonyms:Integrin beta-2, integrin beta chain, beta 2, ITGB2
Isotype:IgG2a k
Reactivity:Human
Applications:FA, FC, IF Microscopy, In Vivo
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Purity:≥98% monomer by analytical SEC ⋅ >95% by SDS Page
Product Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Applications:FA, FC, IF Microscopy, In Vivo
Reactivity:Human
Host Species:Mouse
Specificity:IB4 activity is directed against human CD18 and can also stain rabbit bone marrow leukocytes.
Antigen Distribution:Depending on its heterodimeric configuration, CD18 is expressed in leukocytes, monocytes, macrophages, some lymphocytes, polymorphonuclear lymphocytes (PMNs), natural killer cells, neutrophils, granulocytes, certain lymphocytic tumor cell lines, macrophage foam cells, activated platelets, and splenic red pulp macrophages.
Immunogen:Human peripheral blood lymphocytes
Concentration:≥ 5.0 mg/ml
Endotoxin Level:<0.5 EU/mg as determined by the LAL method
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Purity:≥98% monomer by analytical SEC ⋅ >95% by SDS Page
Preparation:Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.

Integrins are a large family of α/β heterodimeric transmembrane molecules that mediate adhesion, migration, cell survival, and cell differentiation1. The leukocytic cell-adhesion molecule (Leu-CAM) family has three members, LFA-1 (CD18/CD11a), CR3 (CD18/CD11b), and p150,95 (CD18/CD11c), all of which share the β chain CD182.

Murine (m) 1B4 was humanized (h) by the CDR-grafting method to create an anti-CD18 antibody with similar avidity, specificity, and efficacy as the murine antibody3. In rhesus monkeys, h1B4 is less immunogenic than m1B4 and elicits only anti-idiotypic antibodies. Additionally, h1B4 and m1B4 exhibit equivalent staining specificity and intensity in rabbit bone marrow leukocytes.

For humanization, human VH and VL FR were chosen based on high identity with their corresponding m1B4 FR3. Once grafted with the m1B4 CDR, the human VH and VL FR were expressed as fusion proteins with the IgG4 and Cκ human constant regions, respectively.

The h1B4 monoclonal antibody has been used in multiple studies of adhesion in different model systems4, 5, 6 and to measure the expression of CD18 in B-ALL cell lines7.

Protein:CD18
Ligand/Receptor:Receptor
Research Area:Cell Adhesion, Cell Biology, Immunology, Signal Transduction
State of Matter:Liquid
Pathogen Testing:To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Assay Genie's Purified Functional Antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Shipping:2-8°C
NCBI Gene Bank ID:3689
UniProt:P05107
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