Cell Biology Antibodies 5
Anti-HTR1F Antibody (CAB14743)
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- SKU:
- CAB14743
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-HTR1F Antibody |
| Antibody SKU: | CAB14743 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 200-290 of human HTR1F (NP_000857.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 200-290 of human HTR1F (NP_000857.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LYYK IYRA AKTL YHKR QASR IAKE EVNG QVLL ESGE KSTK SVST SYVL EKSL SDPS TDFD KIHS TVRS LRSE FKHE KSWR RQKI SGTR ERK |
| Gene ID: | 3355 |
| Uniprot: | P30939 |
| Cellular Location: | Cell membrane, Multi-pass membrane protein |
| Calculated MW: | 41kDa |
| Observed MW: | 42kDa |
| Synonyms: | HTR1F, 5-HT-1F, 5-HT1F, 5HT6, HTR1EL, MR77 |
| Background: |
| UniProt Protein Function: | 5-HT(1F): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family. |
| UniProt Protein Details: | Protein type:GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 3p12 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:serotonin binding; serotonin receptor activity Biological Process: G-protein signaling, adenylate cyclase inhibiting pathway; G-protein signaling, coupled to cyclic nucleotide second messenger; serotonin receptor signaling pathway; synaptic transmission |
| UniProt Code: | P30939 |
| NCBI GenInfo Identifier: | 398967 |
| NCBI Gene ID: | 3355 |
| NCBI Accession: | P30939.1 |
| UniProt Secondary Accession: | P30939,Q02284, P30940, |
| UniProt Related Accession: | P30939 |
| Molecular Weight: | |
| NCBI Full Name: | 5-hydroxytryptamine receptor 1F |
| NCBI Synonym Full Names: | 5-hydroxytryptamine receptor 1F |
| NCBI Official Symbol: | HTR1F |
| NCBI Official Synonym Symbols: | 5HT6; MR77; 5-HT1F; HTR1EL; 5-HT-1F |
| NCBI Protein Information: | 5-hydroxytryptamine receptor 1F |
| UniProt Protein Name: | 5-hydroxytryptamine receptor 1F |
| UniProt Synonym Protein Names: | Serotonin receptor 1F |
| Protein Family: | 5-hydroxytryptamine receptor |
| UniProt Gene Name: | HTR1F |
| UniProt Entry Name: | 5HT1F_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HTR1F antibody (CAB14743) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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