Cell Biology Antibodies 7
Anti-HTR1D Antibody (CAB1726)
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- SKU:
- CAB1726
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-HTR1D Antibody |
| Antibody SKU: | CAB1726 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human HTR1D (NP_000855.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, SGC-7901, SH-SY5Y, Mouse brain, Mouse testis, Mouse stomach, Rat brain, Rat testis |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 150-250 of human HTR1D (NP_000855.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RTAG HAAT MIAI VWAI SICI SIPP LFWR QAKA QEEM SDCL VNTS QISY TIYS TCGA FYIP SVLL IILY GRIY RAAR NRIL NPPS LYGK RFTT AHLI TGSA G |
| Gene ID: | 3352 |
| Uniprot: | P28221 |
| Cellular Location: | Cell membrane, Multi-pass membrane protein |
| Calculated MW: | 41kDa |
| Observed MW: | 50kDa |
| Synonyms: | HTR1D, 5-HT1D, HT1DA, HTR1DA, HTRL, RDC4 |
| Background: |
| UniProt Protein Function: | 5-HT(1D): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family. |
| UniProt Protein Details: | Protein type:GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR Chromosomal Location of Human Ortholog: 1p36.3-p34.3 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:serotonin receptor activity Biological Process: G-protein signaling, adenylate cyclase inhibiting pathway; G-protein signaling, coupled to cyclic nucleotide second messenger; intestine smooth muscle contraction |
| UniProt Code: | P28221 |
| NCBI GenInfo Identifier: | 112819 |
| NCBI Gene ID: | 3352 |
| NCBI Accession: | P28221.1 |
| UniProt Related Accession: | P28221 |
| Molecular Weight: | 41,907 Da |
| NCBI Full Name: | 5-hydroxytryptamine receptor 1D |
| NCBI Synonym Full Names: | 5-hydroxytryptamine receptor 1D |
| NCBI Official Symbol: | HTR1D |
| NCBI Official Synonym Symbols: | HTRL; RDC4; HT1DA; 5-HT1D; HTR1DA |
| NCBI Protein Information: | 5-hydroxytryptamine receptor 1D |
| UniProt Protein Name: | 5-hydroxytryptamine receptor 1D |
| UniProt Synonym Protein Names: | Serotonin 1D alpha receptor; 5-HT-1D-alpha; Serotonin receptor 1D |
| Protein Family: | 5-hydroxytryptamine receptor |
| UniProt Gene Name: | HTR1D |
| UniProt Entry Name: | 5HT1D_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HTR1D antibody (CAB1726) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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