Epigenetics & Nuclear Signaling Antibodies 3
Anti-HTATSF1 Antibody (CAB14542)
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- SKU:
- CAB14542
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-HTATSF1 Antibody |
| Antibody SKU: | CAB14542 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HTATSF1 (NP_055315.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, Jurkat, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HTATSF1 (NP_055315.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSGT NLDG NDEF DEQL RMQE LYGD GKDG DTQT DAGG EPDS LGQQ PTDT PYEW DLDK KAWF PKIT EDFI ATYQ ANYG FSND GASS STAN VEDV HART AEEP PQEK APEP TDAR KKGE KRKA ESGW FHVE EDRN TNVY VSGL PPDI TVDE FIQL MSKF GIIM RDPQ TEEF KVKL YKDN QGNL |
| Gene ID: | 27336 |
| Uniprot: | O43719 |
| Cellular Location: | Nucleus |
| Calculated MW: | 85kDa |
| Observed MW: | 130kDa |
| Synonyms: | HTATSF1, TAT-SF1, TATSF1, dJ196E23.2 |
| Background: | The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene. |
| UniProt Protein Function: | TAT-SF1: Functions as a general transcription factor playing a role in the process of transcriptional elongation. May mediate the reciprocal stimulatory effect of splicing on transcriptional elongation. In case of infection by HIV-1, it is up-regulated by the HIV-1 proteins NEF and gp120, acts as a cofactor required for the Tat-enhanced transcription of the virus. Belongs to the HTATSF1 family. |
| UniProt Protein Details: | Protein type:Spliceosome; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: Xq26.3 Cellular Component: nucleoplasm; nucleus Molecular Function:nucleotide binding Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; viral genome replication; regulation of RNA elongation |
| NCBI Summary: | The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Sep 2009] |
| UniProt Code: | O43719 |
| NCBI GenInfo Identifier: | 74754346 |
| NCBI Gene ID: | 27336 |
| NCBI Accession: | O43719.1 |
| UniProt Secondary Accession: | O43719,Q59G06, Q99730, D3DWG9, |
| UniProt Related Accession: | O43719 |
| Molecular Weight: | 85,853 Da |
| NCBI Full Name: | HIV Tat-specific factor 1 |
| NCBI Synonym Full Names: | HIV-1 Tat specific factor 1 |
| NCBI Official Symbol: | HTATSF1 |
| NCBI Official Synonym Symbols: | TATSF1; TAT-SF1; dJ196E23.2 |
| NCBI Protein Information: | HIV Tat-specific factor 1; HIV TAT specific factor 1; cofactor required for Tat activation of HIV-1 transcription |
| UniProt Protein Name: | HIV Tat-specific factor 1 |
| Protein Family: | HIV Tat-specific factor |
| UniProt Gene Name: | HTATSF1 |
| UniProt Entry Name: | HTSF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HTATSF1 antibody (CAB14542) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human lung cancer using HTATSF1 antibody (CAB14542) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using HTATSF1 antibody (CAB14542) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using HTATSF1 antibody (CAB14542) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using HTATSF1 antibody (CAB14542) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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