Metabolism Antibodies 2
Anti-HSPE1 Antibody (CAB7437)
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- SKU:
- CAB7437
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-HSPE1 Antibody |
| Antibody SKU: | CAB7437 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-102 of human HSPE1 (NP_002148.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, Mouse liver, Mouse kidney, Mouse heart, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-102 of human HSPE1 (NP_002148.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAGQ AFRK FLPL FDRV LVER SAAE TVTK GGIM LPEK SQGK VLQA TVVA VGSG SKGK GGEI QPVS VKVG DKVL LPEY GGTK VVLD DKDY FLFR DGDI LGKY VD |
| Gene ID: | 3336 |
| Uniprot: | P61604 |
| Cellular Location: | Mitochondrion matrix |
| Calculated MW: | 10kDa |
| Observed MW: | 11kDa |
| Synonyms: | HSPE1, CPN10, EPF, GROES, HSP10 |
| Background: | This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3. |
| UniProt Protein Function: | HSPE1: Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. Belongs to the GroES chaperonin family. |
| UniProt Protein Details: | Protein type:Mitochondrial Chromosomal Location of Human Ortholog: 2q33.1 Cellular Component: membrane; mitochondrial matrix; mitochondrion Molecular Function:ATP binding; chaperone binding; protein binding; unfolded protein binding Biological Process: caspase activation; osteoblast differentiation; protein folding; response to unfolded protein |
| NCBI Summary: | This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb 2011] |
| UniProt Code: | P61604 |
| NCBI GenInfo Identifier: | 47606335 |
| NCBI Gene ID: | 3336 |
| NCBI Accession: | P61604.2 |
| UniProt Secondary Accession: | P61604,O95421, Q04984, Q53X54, Q9UDH0, |
| UniProt Related Accession: | P61604 |
| Molecular Weight: | 10,932 Da |
| NCBI Full Name: | 10 kDa heat shock protein, mitochondrial |
| NCBI Synonym Full Names: | heat shock protein family E (Hsp10) member 1 |
| NCBI Official Symbol: | HSPE1 |
| NCBI Official Synonym Symbols: | EPF; CPN10; GROES; HSP10 |
| NCBI Protein Information: | 10 kDa heat shock protein, mitochondrial |
| UniProt Protein Name: | 10 kDa heat shock protein, mitochondrial |
| UniProt Synonym Protein Names: | 10 kDa chaperonin; Chaperonin 10; CPN10; Early-pregnancy factor; EPF |
| UniProt Gene Name: | HSPE1 |
| UniProt Entry Name: | CH10_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HSPE1 antibody (CAB7437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human gastric using HSPE1 antibody (CAB7437) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using HSPE1 antibody (CAB7437). Blue: DAPI for nuclear staining. |
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