Metabolism Antibodies 2

Anti-HSPB8 Antibody (CAB2514)

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SKU:
CAB2514
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-HSPB8 Antibody
Antibody SKU:CAB2514
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human HSPB8 (NP_055180.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:RD, Mouse liver, Mouse heart, Rat heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-196 of human HSPB8 (NP_055180.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MADG QMPF SCHY PSRL RRDP FRDS PLSS RLLD DGFG MDPF PDDL TASW PDWA LPRL SSAW PGTL RSGM VPRG PTAT ARFG VPAE GRTP PPFP GEPW KVCV NVHS FKPE ELMV KTKD GYVE VSGK HEEK QQEG GIVS KNFT KKIQ LPAE VDPV TVFA SLSP EGLL IIEA PQVP PYST FGES SFNN ELPQ DSQE VTCT
Gene ID:26353
Uniprot:Q9UJY1
Cellular Location:Cytoplasm, Nucleus
Calculated MW:21kDa
Observed MW:22KDa
Synonyms:HSPB8, CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22
Background:The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.
UniProt Protein Function:HSPB8: Displays temperature-dependent chaperone activity. Monomer. Interacts with HSPB1. Interacts with DNAJB6. By 17-beta-estradiol. Predominantly expressed in skeletal muscle and heart. Belongs to the small heat shock protein (HSP20) family.
UniProt Protein Details:

Protein type:Heat shock protein

Chromosomal Location of Human Ortholog: 12q24.23

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; intracellular; nucleus

Molecular Function:identical protein binding; protein binding; protein kinase activity

Disease: Charcot-marie-tooth Disease, Axonal, Type 2l; Neuronopathy, Distal Hereditary Motor, Type Iia

NCBI Summary:The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008]
UniProt Code:Q9UJY1
NCBI GenInfo Identifier:13431576
NCBI Gene ID:26353
NCBI Accession:Q9UJY1.1
UniProt Secondary Accession:Q9UJY1,Q6FIH3, Q9UKS3, B2R6A6,
UniProt Related Accession:Q9UJY1
Molecular Weight:196
NCBI Full Name:Heat shock protein beta-8
NCBI Synonym Full Names:heat shock 22kDa protein 8
NCBI Official Symbol:HSPB8  
NCBI Official Synonym Symbols:H11; HMN2; CMT2L; DHMN2; E2IG1; HMN2A; HSP22  
NCBI Protein Information:heat shock protein beta-8; protein kinase H11; alpha-crystallin C chain; E2-induced gene 1 protein; heat shock 27kDa protein 8; small stress protein-like protein HSP22
UniProt Protein Name:Heat shock protein beta-8
UniProt Synonym Protein Names:Alpha-crystallin C chain; E2-induced gene 1 protein; Protein kinase H11; Small stress protein-like protein HSP22
Protein Family:Heat shock protein
UniProt Gene Name:HSPB8  
UniProt Entry Name:HSPB8_HUMAN
Anti-HSPB8 Antibody (CAB2514)Western blot analysis of extracts of various cell lines, using HSPB8 antibody (CAB2514) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-HSPB8 Antibody (CAB2514)Immunohistochemistry of paraffin-embedded rat heart using HSPB8 Rabbit pAb (CAB2514) at dilution of 1:100 (40x lens).
Anti-HSPB8 Antibody (CAB2514)Immunofluorescence analysis of HeLa cells using HSPB8 antibody (CAB2514). Blue: DAPI for nuclear staining.
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system_update_altDatasheetHuman HSPB8 Recombinant Protein Heat shock protein beta-8 (HSPB8) belongs to the small heat shock protein (HSP20) family. This protein can be inducted by 17-beta-estradi