Antibody Name:	Anti-HSPA1A Antibody (CAB20819)
Antibody SKU:	CAB20819
Antibody Size:	50µL, 100µL
Application:	Western blotting, Immunohistochemistry, Immunofluorescence
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70 (NP_005336.3).

Application:	Western blotting, Immunohistochemistry, Immunofluorescence
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HeLa, A-549, NIH/3T3, C6, Mouse brain, Mouse lung, Rat heart, Rat liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70 (NP_005336.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KITI TNDK GRLS KEEI ERMV QEAE KYKA EDEV QRER VSAK NALE SYAF NMKS AVED EGLK GKIS EADK KKVL DKCQ EVIS WLDA NTLA EKDE FEHK RKEL E
Cellular Location:
Calculated MW:	70kDa
Observed MW:	70KDa

Synonyms:

HEL-S-103, HSP70-1, HSP70-1A, HSP70.1, HSP70I, HSP72, HSPA1

Background:

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. provided by RefSeq, Jul 2008

	Immunofluorescence analysis of PC-12 cells using HSP70 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using HSP70 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of HeLa cells using HSP70 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using HSP70 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of A-549 cells using HSP70 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunohistochemistry of paraffin-embedded human esophageal cancer using HSP70 Rabbit pAb at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using HSP70 Rabbit pAb at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Western blot analysis of extracts of various cell lines, using HSP70 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
	Western blot analysis of extracts of various cell lines, using HSP70 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0. 5s.