Cell Biology Antibodies 10
Anti-HSPA14 Antibody (CAB7107)
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- SKU:
- CAB7107
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-HSPA14 Antibody |
| Antibody SKU: | CAB7107 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 240-509 of human HSPA14 (NP_057383.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | MCF7, SW620, COS-1, HeLa, Jurkat, Mouse spleen, Mouse brain, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 240-509 of human HSPA14 (NP_057383.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ASEF QRSF KHDV RGNA RAMM KLTN SAEV AKHS LSTL GSAN CFLD SLYE GQDF DCNV SRAR FELL CSPL FNKC IEAI RGLL DQNG FTAD DINK VVLC GGSS RIPK LQQL IKDL FPAV ELLN SIPP DEVI PIGA AIEA GILI GKEN LLVE DSLM IECS ARDI LVKG VDES GASR FTVL FPSG TPLP ARRQ HTLQ APGS ISSV CLEL YESD GKNS AKEE TKFA QVVL QDLD KKEN GLRD ILAV LTMK RDGS LHVT CTDQ ETGK CEAI SIEI AS |
| Gene ID: | 51182 |
| Uniprot: | Q0VDF9 |
| Cellular Location: | Cytoplasm, cytosol |
| Calculated MW: | 54kDa |
| Observed MW: | 55kDa |
| Synonyms: | HSPA14, HSP70-4, HSP70L1 |
| Background: |
| UniProt Protein Function: | HSPA14: Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity. Belongs to the heat shock protein 70 family. |
| UniProt Protein Details: | Protein type:Heat shock protein Chromosomal Location of Human Ortholog: 10p13 Cellular Component: cytosol; membrane; ribosome Molecular Function:protein binding Biological Process: 'de novo' cotranslational protein folding |
| UniProt Code: | Q0VDF9 |
| NCBI GenInfo Identifier: | 121948121 |
| NCBI Gene ID: | 51182 |
| NCBI Accession: | Q0VDF9.1 |
| UniProt Secondary Accession: | Q0VDF9,Q9P0X2, Q9UI07, A8K8F8, B0YIY9, |
| UniProt Related Accession: | Q0VDF9 |
| Molecular Weight: | 54,794 Da |
| NCBI Full Name: | Heat shock 70 kDa protein 14 |
| NCBI Synonym Full Names: | heat shock protein family A (Hsp70) member 14 |
| NCBI Official Symbol: | HSPA14 |
| NCBI Official Synonym Symbols: | HSP70-4; HSP70L1 |
| NCBI Protein Information: | heat shock 70 kDa protein 14 |
| UniProt Protein Name: | Heat shock 70 kDa protein 14 |
| UniProt Synonym Protein Names: | HSP70-like protein 1; Heat shock protein HSP60 |
| Protein Family: | Heat shock 70 kDa protein |
| UniProt Gene Name: | HSPA14 |
| UniProt Entry Name: | HSP7E_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HSPA14 antibody (CAB7107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of A549 cells using HSPA14 antibody (CAB7107). Blue: DAPI for nuclear staining. |
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