Cell Biology Antibodies 1

Anti-HSP90B1 Antibody (CAB0989)

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SKU:
CAB0989
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-HSP90B1 Antibody
Antibody SKU:CAB0989
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 550-650 of human HSP90B1 (NP_003290.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:A375, HeLa, LO2, NIH/3T3, Mouse liver
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 550-650 of human HSP90B1 (NP_003290.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ESSP FVER LLKK GYEV IYLT EPVD EYCI QALP EFDG KRFQ NVAK EGVK FDES EKTK ESRE AVEK EFEP LLNW MKDK ALKD KIEK AVVS QRLT ESPC ALVA S
Gene ID:7184
Uniprot:P14625
Cellular Location:Endoplasmic reticulum lumen, Melanosome
Calculated MW:92kDa
Observed MW:110kDa
Synonyms:HSP90B1, ECGP, GP96, GRP94, HEL-S-125m, HEL35, TRA1
Background:This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15.
UniProt Protein Function:GRP94: Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP. Interacts with TLR4 and TLR9, but not with TLR3. Belongs to the heat shock protein 90 family.
UniProt Protein Details:

Protein type:Apoptosis; Heat shock protein; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q24.2-q24.3

Cellular Component: endoplasmic reticulum membrane; focal adhesion; endoplasmic reticulum; endoplasmic reticulum lumen; extracellular region; cytosol; extracellular matrix; membrane; perinuclear region of cytoplasm; plasma membrane; melanosome; midbody; nucleus

Molecular Function:protein binding; low-density lipoprotein receptor binding; RNA binding; unfolded protein binding; virion binding; calcium ion binding; protein phosphatase binding; ATP binding

Biological Process: actin rod formation; receptor-mediated endocytosis; ER-associated protein catabolic process; protein transport; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; unfolded protein response; sequestering of calcium ion; response to hypoxia; toll-like receptor signaling pathway; innate immune response; regulation of phosphoprotein phosphatase activity; negative regulation of apoptosis

NCBI Summary:This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]
UniProt Code:P14625
NCBI GenInfo Identifier:119360
NCBI Gene ID:7184
NCBI Accession:P14625.1
UniProt Secondary Accession:P14625,Q96A97,
UniProt Related Accession:P14625
Molecular Weight:803
NCBI Full Name:Endoplasmin
NCBI Synonym Full Names:heat shock protein 90kDa beta (Grp94), member 1
NCBI Official Symbol:HSP90B1  
NCBI Official Synonym Symbols:ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m  
NCBI Protein Information:endoplasmin; tumor rejection antigen 1; epididymis luminal protein 35; 94 kDa glucose-regulated protein; tumor rejection antigen (gp96) 1; endothelial cell (HBMEC) glycoprotein; heat shock protein 90 kDa beta member 1; stress-inducible tumor rejection antigen gp96; epididymis secretory sperm binding protein Li 125m
UniProt Protein Name:Endoplasmin
UniProt Synonym Protein Names:94 kDa glucose-regulated protein; GRP-94; Heat shock protein 90 kDa beta member 1; Tumor rejection antigen 1; gp96 homolog
Protein Family:Endoplasmin
UniProt Gene Name:HSP90B1  
UniProt Entry Name:ENPL_HUMAN
Anti-HSP90B1 Antibody (CAB0989)Western blot analysis of extracts of various cell lines, using HSP90B1 antibody (CAB0989) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-HSP90B1 Antibody (CAB0989)Immunohistochemistry of paraffin-embedded rat testis using HSP90B1 antibody (CAB0989) at dilution of 1:200 (40x lens).
Anti-HSP90B1 Antibody (CAB0989)Immunohistochemistry of paraffin-embedded human lung cancer using HSP90B1 antibody (CAB0989) at dilution of 1:200 (40x lens).
Anti-HSP90B1 Antibody (CAB0989)Immunohistochemistry of paraffin-embedded mouse kidney using HSP90B1 antibody (CAB0989) at dilution of 1:200 (40x lens).
Anti-HSP90B1 Antibody (CAB0989)Immunofluorescence analysis of HeLa cells using HSP90B1 antibody (CAB0989) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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