KO Validated Antibodies 1

Anti-Hsp90 beta Antibody (CAB18015)[KO Validated]

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SKU:
CAB18015
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Reactivity:
Monkey
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-Hsp90 beta Antibody [KO Validated]
Antibody SKU:CAB18015
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat, Monkey
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Hsp90 beta (NP_031381.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat, Monkey
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Hsp90 beta (NP_031381.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPEE VHHG EEEV ETFA FQAE IAQL MSLI INTF YSNK EIFL RELI SNAS DALD KIRY ESLT DPSK LDSG KELK IDII PNPQ ERTL TLVD TGIG MTKA DLIN NLGT IAKS GTKA FMEA LQAG ADIS MIGQ FGVG FYSA YLVA EKVV VITK HNDD EQYA WESS AGGS FTVR ADHG EPIG RGTK VILH LKED QTEY LEER RVKE VVKK HSQF IGYP ITLY LEKE REKE ISDD EAEE EKGE KEEE DKDD EEKP KIED VGSD EEDD SGKD KKKK TKKI KEKY IDQE ELNK TKPI WTRN PDDI TQEE
Gene ID:3326
Uniprot:P08238
Cellular Location:Cytoplasm, Melanosome
Calculated MW:83kDa
Observed MW:80kDa
Anti-Hsp90 beta Antibody [KO Validated] (CAB18015)Western blot analysis of extracts from normal (control) and HSP90AB1 knockout (KO) HeLa cells, using HSP90AB1 antibody (CAB18015) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Synonyms:D6S182, HSP84, HSP90B, HSPC2, HSPCB, Hsp90 beta, HSP90AB1
Background:This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes.
UniProt Protein Function:HSP90B: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4. Belongs to the heat shock protein 90 family.
UniProt Protein Details:

Protein type:Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: apical plasma membrane; basolateral plasma membrane; brush border membrane; cell surface; cytoplasm; cytosol; inclusion body; melanosome; membrane; mitochondrion; nucleoplasm; signalosome

Molecular Function:ATP binding; dATP binding; double-stranded RNA binding; GTP binding; histone deacetylase binding; kinase binding; nitric-oxide synthase regulator activity; protein binding; protein kinase binding; sulfonylurea receptor binding; TPR domain binding; unfolded protein binding

Biological Process: axon guidance; innate immune response; negative regulation of neuron apoptosis; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; placenta development; positive regulation of cell size; positive regulation of nitric oxide biosynthetic process; positive regulation of protein binding; positive regulation of protein import into nucleus, translocation; protein folding; regulation of protein ubiquitination; response to salt stress; response to unfolded protein; virion attachment to host cell surface receptor

NCBI Summary:This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012]
UniProt Code:P08238
NCBI GenInfo Identifier:17865718
NCBI Gene ID:3326
NCBI Accession:P08238.4
UniProt Secondary Accession:P08238,Q5T9W7, Q9NQW0, Q9NTK6, B2R5P0,
UniProt Related Accession:P08238
Molecular Weight:83,264 Da
NCBI Full Name:Heat shock protein HSP 90-beta
NCBI Synonym Full Names:heat shock protein 90kDa alpha family class B member 1
NCBI Official Symbol:HSP90AB1  
NCBI Official Synonym Symbols:HSP84; HSPC2; HSPCB; D6S182; HSP90B  
NCBI Protein Information:heat shock protein HSP 90-beta
UniProt Protein Name:Heat shock protein HSP 90-beta
UniProt Synonym Protein Names:Heat shock 84 kDa; HSP 84; HSP84
Protein Family:Heat shock protein
UniProt Gene Name:HSP90AB1  
UniProt Entry Name:HS90B_HUMAN
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