Name: Anti-HSD3B1 Antibody (CAB8035)
Brand: Metabolism Antibodies 2
SKU: CAB8035
Availability: OutOfStock

Antibody Name:	HSD3B1 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8035
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human HSD3B1 (NP_000853.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:1000 IF 1:20 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A375

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human HSD3B1 (NP_000853.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTGW SCLV TGAG GFLG QRII RLLV KEKE LKEI RVLD KAFG PELR EEFS KLQN KTKL TVLE GDIL DEPF LKRA CQDV SVII HTAC IIDV FGVT HRES IMNV NVKG TQLL LEAC VQAS VPVF IYTS SIEV AGPN SYKE IIQN GHEE EPLE NTWP APYP HSKK LAEK AVLA ANGW NLKN GGTL YTCA LRPM YIYG EGSR FLSA SINE ALNN NGIL SSVG KFST VNPV YVGN VAWA HILA LRAL QDPK KAPS IRGQ FYYI SDDT PHQS YDNL NYTL SKEF GLRL DSRW SFPL SL
Gene ID:	3283
Uniprot:	P14060
Cellular Location:	Endoplasmic reticulum membrane, Mitochondrion membrane, Single-pass membrane protein
Calculated MW:	42kDa
Observed MW:	50kDa

Synonyms:	HSD3B1, 3BETAHSD, HSD3B, HSDB3, HSDB3A, I, SDR11E1
Background:	The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids.

UniProt Protein Function:	HSD3B1: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17- alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone. Belongs to the 3-beta-HSD family.
UniProt Protein Details:	Protein type:Lipid Metabolism - androgen and estrogen; EC 5.3.3.1; Membrane protein, integral; EC 1.1.1.145; Oxidoreductase; Lipid Metabolism - C21-steroid hormone; Isomerase; Mitochondrial Chromosomal Location of Human Ortholog: 1p13.1 Cellular Component: endoplasmic reticulum membrane; mitochondrial inner membrane; mitochondrial intermembrane space; smooth endoplasmic reticulum membrane Molecular Function:3-beta-hydroxy-delta5-steroid dehydrogenase activity; steroid delta-isomerase activity Biological Process: androgen biosynthetic process; estrogen biosynthetic process; glucocorticoid biosynthetic process; mineralocorticoid biosynthetic process; steroid biosynthetic process
NCBI Summary:	The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids. [provided by RefSeq, Jun 2016]
UniProt Code:	P14060
NCBI GenInfo Identifier:	112767
NCBI Gene ID:	3283
NCBI Accession:	P14060.2
UniProt Secondary Accession:	P14060,Q14545, Q8IV65, A8K691,
UniProt Related Accession:	P14060
Molecular Weight:	42,252 Da
NCBI Full Name:	3 beta-hydroxysteroid dehydrogenase/Delta 5-->
NCBI Synonym Full Names:	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
NCBI Official Symbol:	HSD3B1
NCBI Official Synonym Symbols:	I; HSD3B; HSDB3; HSDB3A; SDR11E1; 3BETAHSD
NCBI Protein Information:	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
UniProt Protein Name:	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
UniProt Synonym Protein Names:	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I; 3-beta-HSD I; Trophoblast antigen FDO161GIncluding the following 2 domains:3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)Alternative name(s):3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase
Protein Family:	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase
UniProt Gene Name:	HSD3B1
UniProt Entry Name:	3BHS1_HUMAN

	Western blot analysis of extracts of A-375 cells, using HSD3B1 antibody (CAB8035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of rat adrenal gland using HSD3B1 Rabbit pAb (CAB8035) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of human placenta using HSD3B1 Rabbit pAb (CAB8035) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse adrenal gland using HSD3B1 Rabbit pAb (CAB8035) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.