Name: Anti-HSD17B6 Antibody (CAB14474)
Brand: Metabolism Antibodies 1
SKU: CAB14474
Availability: OutOfStock

Antibody Name:	Anti-HSD17B6 Antibody
Antibody SKU:	CAB14474
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 178-317 of human HSD17B6 (NP_003716.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	LO2, A-549, DU145, MCF7, HeLa, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 178-317 of human HSD17B6 (NP_003716.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VSKY GVEA FSDI LRRE IQHF GVKI SIVE PGYF RTGM TNMT QSLE RMKQ SWKE APKH IKET YGQQ YFDA LYNI MKEG LLNC STNL NLVT DCME HALT SVHP RTRY SAGW DAKF FFIP LSYL PTSL ADYI LTRS WPKP AQAV
Gene ID:	8630
Uniprot:	O14756
Cellular Location:	Early endosome membrane, Lumenal side, Microsome membrane, Peripheral membrane protein
Calculated MW:	35kDa
Observed MW:	36kDa

Synonyms:	HSD17B6, HSE, RODH, SDR9C6
Background:	The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. The oxidoreductase activity can convert 3 alpha-adiol to dihydrotestosterone, while the epimerase activity can convert androsterone to epi-androsterone. Both reactions use NAD+ as the preferred cofactor. This gene is a member of the retinol dehydrogenase family.

UniProt Protein Function:	HSD17B6: NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3- alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro). Can convert androsterone to epi-androsterone. Androsterone is first oxidized to 5-alpha- androstane-3,17-dione and then reduced to epi-andosterone. Can act on both C-19 and C-21 3-alpha-hydroxysteroids. Belongs to the short-chain dehydrogenases/reductases (SDR) family.
UniProt Protein Details:	Protein type:Oxidoreductase; EC 1.1.1.239; EC 1.1.1.105; EC 1.1.1.62 Chromosomal Location of Human Ortholog: 12q13 Cellular Component: endoplasmic reticulum; early endosome membrane; intracellular Molecular Function:estradiol 17-beta-dehydrogenase activity; electron carrier activity; oxidoreductase activity; catalytic activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity; retinol dehydrogenase activity Biological Process: androgen biosynthetic process; androgen catabolic process
NCBI Summary:	The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. The oxidoreductase activity can convert 3 alpha-adiol to dihydrotestosterone, while the epimerase activity can convert androsterone to epi-androsterone. Both reactions use NAD+ as the preferred cofactor. This gene is a member of the retinol dehydrogenase family. [provided by RefSeq, Aug 2013]
UniProt Code:	O14756
NCBI GenInfo Identifier:	74739479
NCBI Gene ID:	8630
NCBI Accession:	O14756.1
UniProt Related Accession:	O14756
Molecular Weight:	36kDa
NCBI Full Name:	17-beta-hydroxysteroid dehydrogenase type 6
NCBI Synonym Full Names:	hydroxysteroid 17-beta dehydrogenase 6
NCBI Official Symbol:	HSD17B6
NCBI Official Synonym Symbols:	HSE; RODH; SDR9C6
NCBI Protein Information:	17-beta-hydroxysteroid dehydrogenase type 6
UniProt Protein Name:	17-beta-hydroxysteroid dehydrogenase type 6
UniProt Synonym Protein Names:	3-alpha->beta-hydroxysteroid epimerase; 3-alpha->beta-HSE; Oxidative 3-alpha hydroxysteroid dehydrogenase
Protein Family:	17-beta-hydroxysteroid dehydrogenase
UniProt Gene Name:	HSD17B6
UniProt Entry Name:	H17B6_HUMAN

Western blot analysis of extracts of various cell lines, using HSD17B6 antibody (CAB14474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

Anti-HSD17B6 Antibody (CAB14262)

HSD17B6 Antibody (PACO09811)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)