Name: Anti-HSD17B4 Antibody (CAB6187)
Brand: Metabolism Antibodies 2
SKU: CAB6187
Availability: OutOfStock

Antibody Name:	Anti-HSD17B4 Antibody
Antibody SKU:	CAB6187
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 437-736 of human HSD17B4 (NP_000405.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, 22Rv1, HepG2, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 437-736 of human HSD17B4 (NP_000405.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SGVV IIMD VYSY SEKE LICH NQFS LFLV GSGG FGGK RTSD KVKV AVAI PNRP PDAV LTDT TSLN QAAL YRLS GDWN PLHI DPNF ASLA GFDK PILH GLCT FGFS ARRV LQQF ADND VSRF KAIK ARFA KPVY PGQT LQTE MWKE GNRI HFQT KVQE TGDI VISN AYVD LAPT SGTS AKTP SEGG KLQS TFVF EEIG RRLK DIGP EVVK KVNA VFEW HITK GGNI GAKW TIDL KSGS GKVY QGPA KGAA DTTI ILSD EDFM EVVL GKLD PQKA FFSG RLKA RGNI MLSQ KLQM ILKD YAKL
Gene ID:	3295
Uniprot:	P51659
Cellular Location:	Peroxisome
Calculated MW:	77kDa/79kDa/83kDa
Observed MW:	88kDa

Synonyms:

HSD17B4, DBP, MFE-2, MPF-2, PRLTS1, SDR8C1

Background:

The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

UniProt Protein Function:	HSD17B4: Bifunctional enzyme acting on the peroxisomal beta- oxidation pathway for fatty acids. Catalyzes the formation of 3- ketoacyl-CoA intermediates from both straight-chain and 2-methyl- branched-chain fatty acids. Defects in HSD17B4 are a cause of D-bifunctional protein deficiency (DBPD). DBPD is a disorder of peroxisomal fatty acid beta-oxidation. Defects in HSD17B4 are the cause of Perrault syndrome (PRS). PRS is a sex-influenced disorder characterized by sensorineural deafness in both males and females and ovarian dysgenesis in females. Some patients also have neurologic manifestations, including mild mental retardation and cerebellar and peripheral nervous system involvement. Belongs to the short-chain dehydrogenases/reductases (SDR) family.
UniProt Protein Details:	Protein type:EC 4.2.1.107; Lyase; Oxidoreductase; Cell development/differentiation; EC 4.2.1.119; Lipid Metabolism - primary bile acid biosynthesis; EC 1.1.1.n12; Mitochondrial Chromosomal Location of Human Ortholog: 5q21 Cellular Component: intracellular membrane-bound organelle; membrane; peroxisomal matrix; peroxisomal membrane; peroxisome Molecular Function:3-hydroxyacyl-CoA dehydrogenase activity; 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase activity; long-chain-enoyl-CoA hydratase activity; protein homodimerization activity; receptor binding Biological Process: androgen metabolic process; bile acid biosynthetic process; estrogen metabolic process; fatty acid beta-oxidation; fatty acid beta-oxidation using acyl-CoA oxidase; osteoblast differentiation Disease: D-bifunctional Protein Deficiency; Perrault Syndrome 1
NCBI Summary:	The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
UniProt Code:	P51659
NCBI GenInfo Identifier:	1706396
NCBI Gene ID:	3295
NCBI Accession:	P51659.3
UniProt Secondary Accession:	P51659,B4DNV1, B4DVS5, E9PB82, F5HE57,
UniProt Related Accession:	P51659
Molecular Weight:	77,870 Da
NCBI Full Name:	Peroxisomal multifunctional enzyme type 2
NCBI Synonym Full Names:	hydroxysteroid 17-beta dehydrogenase 4
NCBI Official Symbol:	HSD17B4
NCBI Official Synonym Symbols:	DBP; MFE-2; MPF-2; PRLTS1; SDR8C1
NCBI Protein Information:	peroxisomal multifunctional enzyme type 2
UniProt Protein Name:	Peroxisomal multifunctional enzyme type 2
UniProt Synonym Protein Names:	17-beta-hydroxysteroid dehydrogenase 4; 17-beta-HSD 4
UniProt Gene Name:	HSD17B4
UniProt Entry Name:	DHB4_HUMAN

Western blot analysis of extracts of various cell lines, using HSD17B4 antibody (CAB6187) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Antibodies

HSD17B4 Antibody (PACO02072)

HSD17B4 Antibody (PACO09810)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)