Signal Transduction Antibodies 2

Anti-HSCB Antibody (CAB15961)

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SKU:
CAB15961
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-HSCB Antibody
Antibody SKU:CAB15961
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-235 of human HSCB (NP_741999.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:A-549, U-87MG, LO2, SGC-7901
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 30-235 of human HSCB (NP_741999.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AASQ AGSN YPRC WNCG GPWG PGRE DRFF CPQC RALQ APDP TRDY FSLM DCNR SFRV DTAK LQHR YQQL QRLV HPDF FSQR SQTE KDFS EKHS TLVN DAYK TLLA PLSR GLYL LKLH GIEI PERT DYEM DRQF LIEI MEIN EKLA EAES EAAM KEIE SIVK AKQK EFTD NVSS AFEQ DDFE EAKE ILTK MRYF SNIE EKIK LKKI PL
Gene ID:150274
Uniprot:Q8IWL3
Cellular Location:Cytoplasm, Mitochondrion
Calculated MW:27kDa
Observed MW:27kDa
Synonyms:HSCB, DNAJC20, HSC20, JAC1
Background:This gene encodes a DnaJ-type co-chaperone and member of the heat shock cognate B (HscB) family of proteins. The encoded protein plays a role in the synthesis of iron-sulfur clusters, protein cofactors that are involved in the redox reactions of mitochondrial electron transport and other processes. Cells in which this gene is knocked down exhibit reduced activity of iron-sulfur cluster-dependent enzymes including succinate dehydrogenase and aconitase. The encoded protein may stimulate the ATPase activity of the mitochondrial stress-70 protein. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:HSCB: Acts as a co-chaperone in iron-sulfur cluster assembly in mitochondria. Belongs to the HscB family.
UniProt Protein Details:

Protein type:Mitochondrial; Chaperone

Chromosomal Location of Human Ortholog: 22q12.1

Cellular Component: centrosome; mitochondrion; cytoplasm; plasma membrane

Molecular Function:protein binding; chaperone binding; metal ion binding

Biological Process: protein folding; iron-sulfur cluster assembly; protein oligomerization

UniProt Code:Q8IWL3
NCBI GenInfo Identifier:50301234
NCBI Gene ID:150274
NCBI Accession:NP_741999.3
UniProt Secondary Accession:Q8IWL3,Q9BWS7,
UniProt Related Accession:Q8IWL3
Molecular Weight:27,422 Da
NCBI Full Name:iron-sulfur cluster co-chaperone protein HscB, mitochondrial
NCBI Synonym Full Names:HscB mitochondrial iron-sulfur cluster co-chaperone
NCBI Official Symbol:HSCB  
NCBI Official Synonym Symbols:JAC1; HSC20; DNAJC20; dJ366L4.2  
NCBI Protein Information:iron-sulfur cluster co-chaperone protein HscB, mitochondrial; DnaJ (Hsp40) homolog, subfamily C, member 20; HscB iron-sulfur cluster co-chaperone homolog; J-type co-chaperone HSC20; dnaJ homolog subfamily C member 20
UniProt Protein Name:Iron-sulfur cluster co-chaperone protein HscB, mitochondrial
UniProt Synonym Protein Names:DnaJ homolog subfamily C member 20; Hsc20
Protein Family:Co-chaperone protein
UniProt Gene Name:HSCB  
UniProt Entry Name:HSC20_HUMAN
Anti-HSCB Antibody (CAB15961)Western blot analysis of extracts of various cell lines, using HSCB antibody (CAB15961) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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