Cell Biology Antibodies 7

Anti-HS3ST3A1 Antibody (CAB16474)

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SKU:
CAB16474
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-HS3ST3A1 Antibody
Antibody SKU:CAB16474
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 44-140 of human HS3ST3A1 (NP_006033.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-251MG
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 44-140 of human HS3ST3A1 (NP_006033.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ERCQ TLSG PVVG LSGG GEEA GAPG GGVL AGGP RELA VWPA AAQR KRLL QLPQ WRRR RPPA PRDD GEEA AWEE ESPG LSGG PGGS GAGS TVAE APPG T
Gene ID:9955
Uniprot:Q9Y663
Cellular Location:Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:44kDa
Observed MW:45kDa
Synonyms:HS3ST3A1, 3-OST-3A, 3OST3A1
Background:Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It is a type II integral membrane protein and possesses heparan sulfate glucosaminyl 3-O-sulfotransferase activity. The sulfotransferase domain of this enzyme is highly similar to the same domain of heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3B1, and these two enzymes sulfate an identical disaccharide. This gene is widely expressed, with the most abundant expression in liver and placenta.
UniProt Protein Function:HS3ST3A1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N- unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Catalyzes the O-sulfation of glucosamine in IdoUA2S-GlcNS and also in IdoUA2S-GlcNH2. The substrate-specific O-sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes simplex virus-1 (HSV-1) and permits its entry. Unlike 3-OST-1, does not convert non- anticoagulant heparan sulfate to anticoagulant heparan sulfate. Belongs to the sulfotransferase 1 family.
UniProt Protein Details:

Protein type:Glycan Metabolism - heparan sulfate biosynthesis; Membrane protein, integral; EC 2.8.2.23; Glycan Metabolism - glycosaminoglycan degradation; Transferase

Chromosomal Location of Human Ortholog: 17p12

Cellular Component: Golgi membrane; integral to membrane

Molecular Function:[heparan sulfate]-glucosamine 3-sulfotransferase 3 activity; sulfotransferase activity

Biological Process: glycosaminoglycan biosynthetic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis

NCBI Summary:Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It is a type II integral membrane protein and possesses heparan sulfate glucosaminyl 3-O-sulfotransferase activity. The sulfotransferase domain of this enzyme is highly similar to the same domain of heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3A1, and these two enzymes sulfate an identical disaccharide. This gene is widely expressed, with the most abundant expression in liver and placenta. [provided by RefSeq, Jul 2008]
UniProt Code:Q9Y663
NCBI GenInfo Identifier:61214551
NCBI Gene ID:9955
NCBI Accession:Q9Y663.1
UniProt Secondary Accession:Q9Y663,A8K7N2,
UniProt Related Accession:Q9Y663
Molecular Weight:
NCBI Full Name:Heparan sulfate glucosamine 3-O-sulfotransferase 3A1
NCBI Synonym Full Names:heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1
NCBI Official Symbol:HS3ST3A1  
NCBI Official Synonym Symbols:30ST3A1; 3OST3A1  
NCBI Protein Information:heparan sulfate glucosamine 3-O-sulfotransferase 3A1; 3-OST-3A; h3-OST-3A; heparan sulfate 3-O-sulfotransferase 3A1; heparin-glucosamine 3-O-sulfotransferase; heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3A1
UniProt Protein Name:Heparan sulfate glucosamine 3-O-sulfotransferase 3A1
UniProt Synonym Protein Names:Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3A1
Protein Family:Heparan sulfate glucosamine 3-O-sulfotransferase
UniProt Gene Name:HS3ST3A1  
UniProt Entry Name:HS3SA_HUMAN
Anti-HS3ST3A1 Antibody (CAB16474)Western blot analysis of extracts of U-251MG cells, using HS3ST3A1 antibody (CAB16474) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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