Neuroscience

Anti-HRH3 Antibody (CAB16497)

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SKU:
CAB16497
Product Type:
Antibody
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Neuroscience

Description

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Antibody Name:Anti-HRH3 Antibody
Antibody SKU:CAB16497
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 250-350 of human HRH3 (NP_009163.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Rat
Positive Samples:rat brain
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 250-350 of human HRH3 (NP_009163.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PSPP PPPG CWGC WQKG HGEA MPLH RYGV GEAA VGAE AGEA TLGG GGGG GSVA SPTS SSGS SSRG TERP RSLK RGSK PSAS SASL EKRM KMVS QSFT QRFR L
Gene ID:11255
Uniprot:Q9Y5N1
Cellular Location:Cell membrane, Multi-pass membrane protein
Calculated MW:36kDa/40kDa/45kDa/47kDa/48kDa/49kDa
Observed MW:49kDa
Synonyms:HRH3, GPCR97, HH3R
Background:Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene encodes one of the histamine receptors (H3) which belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and can regulate neurotransmitter release. This receptor can also increase voltage-dependent calcium current in smooth muscles and innervates the blood vessels and the heart in cardiovascular system.
UniProt Protein Function:H3R: The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist). Agonist stimulation of isoform 3 niether modified adenylate cyclase activity nor induced intracellular calcium mobilization. Belongs to the G-protein coupled receptor 1 family. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: neuron projection; integral to plasma membrane; plasma membrane; myelin sheath

Molecular Function:drug binding; histamine receptor activity

Biological Process: negative regulation of glutamate secretion; eating behavior; neurotransmitter secretion; drinking behavior; negative regulation of gamma-aminobutyric acid secretion; negative regulation of adenylate cyclase activity; learning; regulation of norepinephrine secretion; response to organic cyclic substance; memory; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of serotonin secretion; elevation of cytosolic calcium ion concentration; brain development; negative regulation of blood pressure; positive regulation of epithelial cell proliferation

NCBI Summary:Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by histamine receptors H1, H2, H3 and H4. This gene encodes one of the histamine receptors (H3) which belongs to the family 1 of G protein-coupled receptors. It is an integral membrane protein and can regulate neurotransmitter release. This receptor can also increase voltage-dependent calcium current in smooth muscles and innervates the blood vessels and the heart in cardiovascular system. [provided by RefSeq, Jul 2008]
UniProt Code:Q9Y5N1
NCBI GenInfo Identifier:17367264
NCBI Gene ID:11255
NCBI Accession:Q9Y5N1.2
UniProt Secondary Accession:Q9Y5N1,Q4QRI7, Q9GZX2, Q9H4K8,
UniProt Related Accession:Q9Y5N1
Molecular Weight:45,607 Da
NCBI Full Name:Histamine H3 receptor
NCBI Synonym Full Names:histamine receptor H3
NCBI Official Symbol:HRH3  
NCBI Official Synonym Symbols:HH3R; GPCR97  
NCBI Protein Information:histamine H3 receptor; G protein-coupled receptor 97; G-protein coupled receptor 97; H3R
UniProt Protein Name:Histamine H3 receptor
UniProt Synonym Protein Names:G-protein coupled receptor 97
Protein Family:Histamine H3 receptor
UniProt Gene Name:HRH3  
UniProt Entry Name:HRH3_HUMAN
Anti-HRH3 Antibody (CAB16497)Western blot analysis of extracts of rat brain, using HRH3 antibody (CAB16497) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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